| Literature DB >> 27665558 |
Marcos Vinicios Salles Dias1, Vilma Regina Martins1, Glaucia Noeli Maroso Hajj2.
Abstract
This chapter is derived from our experience in the study of stress-Inducible Protein 1 (STI1) in extracellular vesicles. We used different techniques to isolate, explore, and characterize the extracellular vesicles that contained this protein. Ultracentrifugation and gel chromatography were used to isolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles.Entities:
Keywords: Extracellular vesicles; Gel chromatography; Nanoparticle tracking analysis (NTA); Stress-Inducible Protein 1 (STI1); Ultracentrifugation
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Year: 2016 PMID： 27665558 DOI： 10.1007/978-1-4939-3804-9_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745