Zhi-Ping Guo1, Tao Wang2, Lan-Ping Xu1, Xiao-Hui Zhang1, Yu Wang1, Xiao-Jun Huang1, Ying-Jun Chang3. 1. Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China. 2. Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China; Department of Hematology, Shanxi Da Yi Hospital of Shanxi Medical University, Taiyuan, Shanxi 030032, China. 3. Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China; Collaborative Innovation Center of Hematology, Peking University, Beijing 100044, China. Electronic address: rmcyj@bjmu.edu.cn.
Abstract
BACKGROUND: A second allogeneic hematopoietic stem-cell transplantation and donor lymphocyte infusion using cells from the same donor is a therapeutic option in the case of stem-cell graft failure or disease relapse, but little is known about the factors associated with the CD34+ cell yields from second donations. METHODS: One-hundred healthy donors who underwent a second mobilization treatment and peripheral blood stem-cell (PBSC) collection were studied. For both mobilization processes, 5 µg of granulocyte colony-stimulating factor per kg per day was administered. The blood counts of the donors were monitored during the processes. RESULTS: The second donations from the same donors provided lower apheresis yields than did the initial collections. The number of CD34+ cells collected from normal donors after a second cycle of PBSC mobilization was associated with their steady-state lymphocyte counts and the intertransplantation interval. Female sex negatively affected the CD34+ cell yields. The cutoff value for the steady-state absolute lymphocyte count was 2.055 × 109/L. CONCLUSION: To harvest greater numbers of CD34+ cells from second collections, male donors and those with intervals of longer than 9 months between donations should be selected. The lymphocyte counts prior to the first donations may predict the content of CD34+ cells in the allografts prepared using the second donations.
BACKGROUND: A second allogeneic hematopoietic stem-cell transplantation and donor lymphocyte infusion using cells from the same donor is a therapeutic option in the case of stem-cell graft failure or disease relapse, but little is known about the factors associated with the CD34+ cell yields from second donations. METHODS: One-hundred healthy donors who underwent a second mobilization treatment and peripheral blood stem-cell (PBSC) collection were studied. For both mobilization processes, 5 µg of granulocyte colony-stimulating factor per kg per day was administered. The blood counts of the donors were monitored during the processes. RESULTS: The second donations from the same donors provided lower apheresis yields than did the initial collections. The number of CD34+ cells collected from normal donors after a second cycle of PBSC mobilization was associated with their steady-state lymphocyte counts and the intertransplantation interval. Female sex negatively affected the CD34+ cell yields. The cutoff value for the steady-state absolute lymphocyte count was 2.055 × 109/L. CONCLUSION: To harvest greater numbers of CD34+ cells from second collections, male donors and those with intervals of longer than 9 months between donations should be selected. The lymphocyte counts prior to the first donations may predict the content of CD34+ cells in the allografts prepared using the second donations.
Authors: Sandhya R Panch; Brent Logan; Jennifer A Sees; Stephanie Bo-Subait; Bipin Savani; Nirali N Shah; Jack W Hsu; Galen Switzer; Hillard M Lazarus; Paolo Anderlini; Peiman Hematti; Dennis Confer; Michael A Pulsipher; Bronwen E Shaw; David F Stroncek Journal: Transplant Cell Ther Date: 2021-03-09