Complete Genome Sequence of Plesiomonas shigelloides Type Strain NCTC10360.

Plesiomonas shigelloides is a Gram-negative rod within the Enterobacteriaceae family. It is a gastrointestinal pathogen of increasing notoriety, often associated with diarrheal disease. P. shigelloides is waterborne, and infection is often linked to the consumption of seafood. Here, we describe the first complete genome for P. shigelloides type strain NCTC10360. © Crown copyright 2016.

GENOME ANNOUNCEMENT

Plesiomonas shigelloides is a flagellated, facultative anaerobic, oxidase-positive, and rod-shaped bacterium which has recently been classified within the Enterobacteriaceae family. P. shigelloides is the only species in the Plesiomonas genus, and this species historically has been classified in the Vibrionaceae family and the Aeromonas genus (1, 2). To date, this bacterium has been associated mainly with aquatic environments and organisms that reside in aquatic environments. P. shigelloides has been isolated from streams, lakes, estuarine waters, mammals, crustaceans, mollusks, reptiles, amphibians, and fish (3).

P. shigelloides is a known human pathogen of increasing significance. Principally, it has been linked to gastrointestinal infection, mainly diarrheal diseases, and also in rarer isolated cases to invasive infections, including sepsis, meningitis, and pseudoappendicitis (4). It is possible that cases of P. shigelloides are underreported, because although this bacterial species can be identified using a range of commercially available biochemical test systems (Vitex 2 system and Phoenix 100 ID/AST NID card), its low abundance in fecal samples and small colony size can result in its presence being overlooked (4). To date, there is only one other draft genome sequence available for P. shigelloides, that from strain 302-73. This sequence, however, is composed of 13 scaffolds containing 389 contigs (5). Here, we report the first available complete genome sequence of P. shigelloides from the type strain NCTC10360.

Genomic DNA was extracted from 24-h pure culture using the MasterPure DNA extraction kit (Epicentre, WI, USA). Whole-genome sequencing (WGS) was performed using the Pacific Bioscience (PacBio) RSII platform. DNA was sheared to 15 kb, and 10 kb- to 20 kb-library and sequencing were performed utilizing C4/P6 chemistry. Genome assembly was performed using an automated assembly pipeline (HGAP/Quiver software package), and annotation was performed with Prokka.

The genome of strain NCTC10360 was 3,405,979 bp, which was confined to a single chromosome; no plasmids were identified in this strain. The average G+C content of the sequence was 52%. In total, there were 3,093 coding sequences, including 11 rRNA operons and 113 tRNA genes.

Accession number(s).

The complete genome sequence has been deposited to the European Nucleotide Archive under accession number LT575468.