Literature DB >> 27654995

Organoid Culture of Human Cancer Stem Cells.

Yohei Shimono1, Junko Mukohyama2, Taichi Isobe3, Darius M Johnston4, Piero Dalerba5, Akira Suzuki2.   

Abstract

Organoid culture is a three-dimensional culture method that enables ex vivo analysis of stem cell behavior and differentiation. This method is also applicable to the studies on stem cell characters of human cancer stem cells. The components of organoid culture include Matrigel® and a culture medium containing growth factor cocktails that mimic the microenvironments of organ stem cell niches. Here, we describe the basic methods for the organoid culture of dissociated or FACS-sorted human cancer stem cells. Then, we introduce a method to dissociate the organoids for serial passage and propagation.

Entities:  

Keywords:  Cancer stem cells; Clonogenicity; Growth factor cocktails; Matrigel; Organoids; Passage

Mesh：

Year:  2019        PMID： 27654995     DOI： 10.1007/7651_2016_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  miR-221 Targets QKI to Enhance the Tumorigenic Capacity of Human Colorectal Cancer Stem Cells.

Authors:  Junko Mukohyama; Taichi Isobe; Qingjiang Hu; Takanori Hayashi; Takashi Watanabe; Masao Maeda; Hisano Yanagi; Xin Qian; Kimihiro Yamashita; Hironobu Minami; Koshi Mimori; Debashis Sahoo; Yoshihiro Kakeji; Akira Suzuki; Piero Dalerba; Yohei Shimono
Journal:  Cancer Res       Date:  2019-08-15       Impact factor: 12.701

2.  Upregulation of BMI1-suppressor miRNAs (miR-200c, miR-203) during terminal differentiation of colon epithelial cells.

Authors:  Shigeo Hisamori; Junko Mukohyama; Sanjay Koul; Takanori Hayashi; Piero Dalerba; Yohei Shimono; Michael Evan Rothenberg; Masao Maeda; Taichi Isobe; Luis Enrique Valencia Salazar; Xin Qian; Darius Michael Johnston; Dalong Qian; Kaiqin Lao; Naoya Asai; Yoshihiro Kakeji; Vincenzo Alessandro Gennarino; Debashis Sahoo
Journal:  J Gastroenterol       Date:  2022-03-04       Impact factor: 6.772

3.  MiR-3622a-3p acts as a tumor suppressor in colorectal cancer by reducing stemness features and EMT through targeting spalt-like transcription factor 4.

Authors:  Shuchen Chang; Guangli Sun; Dan Zhang; Qing Li; Haihua Qian
Journal:  Cell Death Dis       Date:  2020-07-27       Impact factor: 8.469

4.  The paracrine induction of prostate cancer progression by caveolin-1.

Authors:  Chun-Jung Lin; Eun-Jin Yun; U-Ging Lo; Yu-Ling Tai; Su Deng; Elizabeth Hernandez; Andrew Dang; Yu-An Chen; Debabrata Saha; Ping Mu; Ho Lin; Tsai-Kun Li; Tang-Long Shen; Chih-Ho Lai; Jer-Tsong Hsieh
Journal:  Cell Death Dis       Date:  2019-11-04       Impact factor: 8.469

5.  Upregulation of S100A10 in metastasized breast cancer stem cells.

Authors:  Hisano Yanagi; Takashi Watanabe; Tatsunori Nishimura; Takanori Hayashi; Seishi Kono; Hitomi Tsuchida; Munetsugu Hirata; Yuko Kijima; Shintaro Takao; Seiji Okada; Motoshi Suzuki; Kazuyoshi Imaizumi; Kenji Kawada; Hironobu Minami; Noriko Gotoh; Yohei Shimono
Journal:  Cancer Sci       Date:  2020-10-11       Impact factor: 6.716
  5 in total

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