| Literature DB >> 27654651 |
David W Greening, Sze Ting Lee, Hong Ji, Richard J Simpson, Angela Rigopoulos, Carmel Murone, Catherine Fang, Sylvia Gong, Graeme O'Keefe, Andrew M Scott.
Abstract
Entities:
Year: 2016 PMID: 27654651 PMCID: PMC5085247 DOI: 10.18632/oncotarget.9855
Source DB: PubMed Journal: Oncotarget ISSN: 1949-2553
Figure 5Validation of anti-tumour effects of cetuximab and bevacizumab mediated through altered cellular metabolism
Proteins were extracted from tumour xenografts for each treatment cohort (independent from the tumour xenograft lysates performed for proteomic profiling), obtained from pooled tumour xenograft samples from the validation experimental group (n=3). Immunoblotting analysis of the expression of ATP5B, SDC2, GPD2, ARF4 in both LIM1215 and HT-29 tumour xenograft lysates was performed (n=3; pooled for each treatment cohort, independent biological replicates performed for each antibody). Loading control (GAPDH; rabbit anti-GAPDH (Cell Signaling Technology; 1:1000)).