Ivana Musilova1, Ctirad Andrys2, Marcela Drahosova2, Ondrej Soucek2, Lenka Pliskova3, Martin Stepan1, Tomas Bestvina1, Jan Maly4, Bo Jacobsson5,6, Marian Kacerovsky1,7. 1. a Department of Obstetrics and Gynecology. 2. b Department of Clinical Immunology and Allergy. 3. c Institute of Clinical Biochemistry and Diagnostics. 4. d Department of Pediatrics , Charles University in Prague, Faculty of Medicine, Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic. 5. e Department of Obstetrics and Gynecology , Sahlgrenska Academy, Gothenburg University , Gothenburg , Sweden. 6. f Department of Genetics and Bioinformatics, Division of Health Data and Digitalization , Norwegian Institute of Public Health , Oslo , Norway , and. 7. g Biomedical Research Center, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Abstract
OBJECTIVE: The aim of this study was to evaluate the amniotic fluid cathepsin-G concentrations in women with preterm prelabor rupture of membranes (PPROM) based on the presence of the microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). METHODS: A total of 154 women with singleton pregnancies complicated by PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid cathepsin-G concentrations were assessed by ELISA. MIAC was determined using a non-cultivation approach. IAI was defined as an amniotic fluid bedside interleukin-6 concentration ≥ 745 pg/mL. RESULTS: Women with MIAC had higher amniotic fluid cathepsin-G concentrations than women without MIAC (with MIAC: median 82.7 ng/mL, versus without MIAC: median 64.7 ng/mL; p = 0.0003). Women with IAI had higher amniotic fluid cathepsin-G concentrations than women without this complication (with IAI: median 103.0 ng/mL, versus without IAI: median 66.2 ng/mL; p < 0.0001). Women with microbial-associated (with both MIAC and IAI) IAI and sterile (IAI without MIAC) IAI had higher amniotic fluid cathepsin-G concentrations than women with colonization (MIAC without IAI) and women without both MIAC and IAI (p < 0.0001). CONCLUSIONS: The presence of either microbial-associated or sterile IAI was associated with increased amniotic fluid cathepsin-G concentrations in pregnancies complicated by PPROM. Amniotic fluid cathepsin-G appears to be a potential marker of IAI.
OBJECTIVE: The aim of this study was to evaluate the amniotic fluid cathepsin-G concentrations in women with preterm prelabor rupture of membranes (PPROM) based on the presence of the microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). METHODS: A total of 154 women with singleton pregnancies complicated by PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid cathepsin-G concentrations were assessed by ELISA. MIAC was determined using a non-cultivation approach. IAI was defined as an amniotic fluid bedside interleukin-6 concentration ≥ 745 pg/mL. RESULTS:Women with MIAC had higher amniotic fluid cathepsin-G concentrations than women without MIAC (with MIAC: median 82.7 ng/mL, versus without MIAC: median 64.7 ng/mL; p = 0.0003). Women with IAI had higher amniotic fluid cathepsin-G concentrations than women without this complication (with IAI: median 103.0 ng/mL, versus without IAI: median 66.2 ng/mL; p < 0.0001). Women with microbial-associated (with both MIAC and IAI) IAI and sterile (IAI without MIAC) IAI had higher amniotic fluid cathepsin-G concentrations than women with colonization (MIAC without IAI) and women without both MIAC and IAI (p < 0.0001). CONCLUSIONS: The presence of either microbial-associated or sterile IAI was associated with increased amniotic fluid cathepsin-G concentrations in pregnancies complicated by PPROM. Amniotic fluid cathepsin-G appears to be a potential marker of IAI.
Authors: Jose Galaz; Roberto Romero; Yi Xu; Derek Miller; Dustyn Levenson; Robert Para; Aneesha Varrey; Richard Hsu; Anna Tong; Sonia S Hassan; Chaur-Dong Hsu; Nardhy Gomez-Lopez Journal: J Perinat Med Date: 2020-09-25 Impact factor: 1.901
Authors: Ivana Musilova; Lenka Pliskova; Romana Gerychova; Petr Janku; Ondrej Simetka; Petr Matlak; Bo Jacobsson; Marian Kacerovsky Journal: PLoS One Date: 2017-12-12 Impact factor: 3.240
Authors: Ivana Musilova; Marian Kacerovsky; Martin Stepan; Tomas Bestvina; Lenka Pliskova; Barbora Zednikova; Bo Jacobsson Journal: PLoS One Date: 2017-08-16 Impact factor: 3.240
Authors: Megan Hall; Jana Hutter; Natalie Suff; Carla Avena Zampieri; Rachel M Tribe; Andrew Shennan; Mary Rutherford; Lisa Story Journal: Prenat Diagn Date: 2022-06-17 Impact factor: 3.242
Authors: Hassendrini N Peiris; Roberto Romero; Kanchan Vaswani; Sarah Reed; Nardhy Gomez-Lopez; Adi L Tarca; Dereje W Gudicha; Offer Erez; Eli Maymon; Murray D Mitchell Journal: J Matern Fetal Neonatal Med Date: 2019-12-29