Literature DB >> 27650198

A simplified mycobacterial growth inhibition assay (MGIA) using direct infection of mouse splenocytes and the MGIT system.

Amy L Yang1, Thomas E Schmidt1, Scott Stibitz1, Steven C Derrick2, Sheldon L Morris1, Marcela Parra1.   

Abstract

We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for pre-clinical assessment of vaccine-mediated protection in mice. The assay is accomplished by directly infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated immunogenicity detected by this assay correlated with protection. Published by Elsevier B.V.

Entities:  

Keywords:  BCG; MGIA; MGIT; Tuberculosis; Vaccine

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Year:  2016        PMID： 27650198     DOI： 10.1016/j.mimet.2016.09.010

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  5 in total

1.  Mycobacterial growth inhibition is associated with trained innate immunity.

Authors:  Simone A Joosten; Krista E van Meijgaarden; Sandra M Arend; Corine Prins; Fredrik Oftung; Gro Ellen Korsvold; Sandra V Kik; Rob Jw Arts; Reinout van Crevel; Mihai G Netea; Tom Hm Ottenhoff
Journal:  J Clin Invest       Date:  2018-04-03       Impact factor: 14.808

2.  Optimisation of a murine splenocyte mycobacterial growth inhibition assay using virulent Mycobacterium tuberculosis.

Authors:  Christina Jensen; Line Lindebo Holm; Erik Svensson; Claus Aagaard; Morten Ruhwald
Journal:  Sci Rep       Date:  2017-06-06       Impact factor: 4.379

Review 3.  The Cross-Species Mycobacterial Growth Inhibition Assay (MGIA) Project, 2010-2014.

Authors:  Michael J Brennan; Rachel Tanner; Sheldon Morris; Thomas J Scriba; Jacqueline M Achkar; Andrea Zelmer; David A Hokey; Angelo Izzo; Sally Sharpe; Ann Williams; Adam Penn-Nicholson; Mzwandile Erasmus; Elena Stylianou; Daniel F Hoft; Helen McShane; Helen A Fletcher
Journal:  Clin Vaccine Immunol       Date:  2017-09-05

4.  The in vitro direct mycobacterial growth inhibition assay (MGIA) for the early evaluation of TB vaccine candidates and assessment of protective immunity: a protocol for non-human primate cells.

Authors:  Rachel Tanner; Emily Hoogkamer; Julia Bitencourt; Andrew White; Charelle Boot; Claudia C Sombroek; Stephanie A Harris; Matthew K O'Shea; Daniel Wright; Rachel Wittenberg; Charlotte Sarfas; Iman Satti; Frank A W Verreck; Sally A Sharpe; Helen A Fletcher; Helen McShane
Journal:  F1000Res       Date:  2021-03-30

5.  A non-human primate in vitro functional assay for the early evaluation of TB vaccine candidates.

Authors:  Rachel Tanner; Andrew D White; Charelle Boot; Claudia C Sombroek; Matthew K O'Shea; Daniel Wright; Emily Hoogkamer; Julia Bitencourt; Stephanie A Harris; Charlotte Sarfas; Rachel Wittenberg; Iman Satti; Helen A Fletcher; Frank A W Verreck; Sally A Sharpe; Helen McShane
Journal:  NPJ Vaccines       Date:  2021-01-04       Impact factor: 7.344
  5 in total

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