Literature DB >> 2764966

Reconstitution of C27-steroid 26-hydroxylase activity from bovine brain mitochondria.

J I Pedersen1, H Oftebro, I Björkhem.   

Abstract

We have previously demonstrated the presence of cytochrome P-450 in a soluble preparation of bovine brain mitochondria (Oftebro, H., Størmer, F.C., and Pedersen, J.I. (1979) J. Biol. Chem. 254, 4331). In the present work we show that this preparation, in the presence of NADPH, adrenodoxin and adrenodoxin reductase catalyzes omega-hydroxylation of a number of C27-steroids that are intermediates in bile acid biosynthesis. The rates of hydroxylation were 1-2 order of magnitudes lower than reported for similar preparations from rat and human liver. No significant activity was detected with cholesterol as substrate. The physiological significance of brain mitochondrial cytochrome P-450 is discussed.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2764966

Source DB:  PubMed          Journal:  Biochem Int        ISSN： 0158-5231


  6 in total

Review 1.  Review of progress in sterol oxidations: 1987-1995.

Authors:  L L Smith
Journal:  Lipids       Date:  1996-05       Impact factor: 1.880

2.  Metabolism of 27-, 25- and 24-hydroxycholesterol in rat glial cells and neurons.

Authors:  J Zhang; Y Akwa; M el-Etr; E E Baulieu; J Sjövall
Journal:  Biochem J       Date:  1997-02-15       Impact factor: 3.857

3.  Cholesterol homeostasis in human brain: evidence for an age-dependent flux of 24S-hydroxycholesterol from the brain into the circulation.

Authors:  D Lütjohann; O Breuer; G Ahlborg; I Nennesmo; A Sidén; U Diczfalusy; I Björkhem
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-03       Impact factor: 11.205

4.  Lack of 3 beta-hydroxy-delta 5-C27-steroid dehydrogenase/isomerase in fibroblasts from a child with urinary excretion of 3 beta-hydroxy-delta 5-bile acids. A new inborn error of metabolism.

Authors:  M S Buchmann; E A Kvittingen; H Nazer; T Gunasekaran; P T Clayton; J Sjövall; I Björkhem
Journal:  J Clin Invest       Date:  1990-12       Impact factor: 14.808

5.  Atherosclerosis and sterol 27-hydroxylase: evidence for a role of this enzyme in elimination of cholesterol from human macrophages.

Authors:  I Björkhem; O Andersson; U Diczfalusy; B Sevastik; R J Xiu; C Duan; E Lund
Journal:  Proc Natl Acad Sci U S A       Date:  1994-08-30       Impact factor: 11.205

6.  Liquid chromatography-mass spectrometry utilizing multi-stage fragmentation for the identification of oxysterols.

Authors:  Kersti Karu; Martin Hornshaw; Gary Woffendin; Karl Bodin; Mats Hamberg; Gunvor Alvelius; Jan Sjövall; John Turton; Yuqin Wang; William J Griffiths
Journal:  J Lipid Res       Date:  2007-01-24       Impact factor: 5.922
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.