| Literature DB >> 27648370 |
Wesley K Utomo1, Vincent T Janmaat1, Auke P Verhaar1, Jérôme Cros2, Philippe Lévy3, Philippe Ruszniewski3, Mirella S Vredenbregt-van den Berg4, Guido Jenster4, Marco J Bruno1, Henri Braat1, Gwenny M Fuhler1, Maikel P Peppelenbosch1.
Abstract
Identification of pancreatic cysts with malignant potential is important to prevent pancreatic cancer development. Integrity of cell free DNA (cfDNA) has been described as tumor biomarker, but its potential for pancreatic cancer is unclear. While normal apoptotic cells release uniformly truncated DNA, malignant tissues release long fragments of cell free DNA (cfDNA). We measured 247 base pair (bp) and 115 bp DNA fragments of ALU repeats by qPCR in serum from healthy controls and pancreatic cancer patients, and in cyst fluid from pancreatic cyst patients. No differences in total cfDNA (ALU115) and cfDNA integrity (ALU247/115) were observed between sera from healthy controls (n=19) and pancreatic cancer patients (n=19). Although elevated as compared to serum, but no differences in cfDNA were found in cyst fluid from high risk (n=10) and low risk (n=20) cyst patients. We conclude that cfDNA integrity is not a useful marker to identify (pre)malignant pancreatic lesions.Entities:
Keywords: ALU repeats; Cell free DNA; PCR; circulating; pancreatic cyst fluid; serum
Year: 2016 PMID： 27648370 PMCID： PMC5004084
Source DB: PubMed Journal: Am J Cancer Res ISSN： 2156-6976 Impact factor: 6.166