Literature DB >> 27644970

Stable isotope dimethyl labelling for quantitative proteomics and beyond.

Jue-Liang Hsu1, Shu-Hui Chen2.   

Abstract

Stable-isotope reductive dimethylation, a cost-effective, simple, robust, reliable and easy-to- multiplex labelling method, is widely applied to quantitative proteomics using liquid chromatography-mass spectrometry. This review focuses on biological applications of stable-isotope dimethyl labelling for a large-scale comparative analysis of protein expression and post-translational modifications based on its unique properties of the labelling chemistry. Some other applications of the labelling method for sample preparation and mass spectrometry-based protein identification and characterization are also summarized.This article is part of the themed issue 'Quantitative mass spectrometry'.
© 2016 The Author(s).

Entities:  

Keywords:  LC-MS/MS; dimethyl labelling; post-translational modifications; quantitative proteomics; stable-isotope labelling

Mesh:

Year:  2016        PMID: 27644970      PMCID: PMC5031631          DOI: 10.1098/rsta.2015.0364

Source DB:  PubMed          Journal:  Philos Trans A Math Phys Eng Sci        ISSN: 1364-503X            Impact factor:   4.226


  103 in total

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