| Literature DB >> 27644247 |
Wanjing Shen1, Yanyan Yuan1, Ming Zhao1, Jiahui Li1, Jin Xu2, Guoying Lou1, Jiachen Zheng1, Shizhong Bu1, Junming Guo1, Yang Xi3.
Abstract
Long non-coding RNAs (lncRNAs) play an important role in cancer occurrence and development. We previously demonstrated that lncRNA gastric cancer-associated transcript 3 (GACAT3) was positively correlated with TNM stages, tumor size, and distant metastasis of patients with gastric cancer. However, the role of GACAT3 in gastric cancer remains unclear. In this study, to investigate its function, we synthesized small interference RNAs (siRNAs) against GACTA3 and developed a GACAT3 overexpression vector (pcDNA3-GACAT3), respectively. The siRNA-mediated knockdown of GACAT3 significantly decreased cell proliferation of the gastric cancer HGC-27 cells, in which GACAT3 is overexpressed. Furthermore, GACAT3 overexpression in gastric cancer SGC-7901 cells promoted cell growth. Moreover, GACAT3 expression in HGC-27 cells was greatly upregulated by IL-6 treatment in a concentration-dependent manner. In contrast, siRNA-mediated knockdown of STAT3 decreased GACAT3 expression even in the presence of IL-6. These results demonstrated that as a downstream target of the IL6/STAT3 signaling, lncRNA GACAT3 promotes gastric cancer cell growth suggesting that GACAT3 is an inflammatory response gene and may be served as a valuable potential target for the treatment of gastric cancer.Entities:
Keywords: GACAT3; Gastric cancer; IL-6; LncRNA; STAT3
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Year: 2016 PMID： 27644247 DOI： 10.1007/s13277-016-5372-8
Source DB: PubMed Journal: Tumour Biol ISSN： 1010-4283