Yang Yu1, Wang Mingjiao2, Xiaohui Yang2, Miao Sui2, Tao Zhang2, Jin Liang3, Xinyi Gu3, Xiaomei Wang2. 1. Endocrinology department of Affiliated Zhongshan Hospital of Dalian University, 6 JieFang Street, Zhongshan District, DaLian, 116001 Liaoning, PR China. Electronic address: someso@sina.com. 2. Endocrinology department of Affiliated Zhongshan Hospital of Dalian University, 6 JieFang Street, Zhongshan District, DaLian, 116001 Liaoning, PR China. 3. Neurology department of Affiliated Zhongshan Hospital of Dalian University, DaLian, 116001 Liaoning, PR China.
Abstract
OBJECTIVES: In the present study, we examined whether DNA methylation of the sortilin-related receptor 1 (SORL1) 5'-flanking region is associated with the manifestation and clinical presentation of mild cognitive impairment in type 2 diabetes mellitus (T2DM). METHODS: Of 84 diabetic patients with mild cognitive impairment (MCI group), 78 diabetic patients without mild cognitive impairment (NMCI group) and 80 age-matched normal controls (NC group), the DNA methylation of the SORL1 5'-flanking region was completely analyzed. The SORL1 methylation ratios of the above three groups were compared statistically. Next, we investigated the correlation between the DNA methylation status and the clinical presentation of diabetes with or without cognitive impairment (MCI and NMCI groups). RESULTS: The methylation ratio (86.9%) of MCI patients was significantly higher than that in the NMCI patients (35.9%, P<0.05) and in the NC group (11.3%, P<0.05). Moreover, the diabetic patients with methylation alleles had greater ages, longer diabetes duration, lower MOCA scores and higher plasma amyloid Aβ 1-42 levels than those with unmethylation alleles (P<0.05). CONCLUSION: These results suggested that the DNA methylation of the SORL1 5'-flanking region may significantly influence the manifestation of mild cognitive impairment in T2DM, and might be associated with its neurocognitive presentation. Copyright Â
OBJECTIVES: In the present study, we examined whether DNA methylation of the sortilin-related receptor 1 (SORL1) 5'-flanking region is associated with the manifestation and clinical presentation of mild cognitive impairment in type 2 diabetes mellitus (T2DM). METHODS: Of 84 diabeticpatients with mild cognitive impairment (MCI group), 78 diabeticpatients without mild cognitive impairment (NMCI group) and 80 age-matched normal controls (NC group), the DNA methylation of the SORL1 5'-flanking region was completely analyzed. The SORL1 methylation ratios of the above three groups were compared statistically. Next, we investigated the correlation between the DNA methylation status and the clinical presentation of diabetes with or without cognitive impairment (MCI and NMCI groups). RESULTS: The methylation ratio (86.9%) of MCI patients was significantly higher than that in the NMCI patients (35.9%, P<0.05) and in the NC group (11.3%, P<0.05). Moreover, the diabeticpatients with methylation alleles had greater ages, longer diabetes duration, lower MOCA scores and higher plasma amyloid Aβ 1-42 levels than those with unmethylation alleles (P<0.05). CONCLUSION: These results suggested that the DNA methylation of the SORL1 5'-flanking region may significantly influence the manifestation of mild cognitive impairment in T2DM, and might be associated with its neurocognitive presentation. Copyright Â
Authors: Anna Morozova; Yana Zorkina; Olga Abramova; Olga Pavlova; Konstantin Pavlov; Kristina Soloveva; Maria Volkova; Polina Alekseeva; Alisa Andryshchenko; Georgiy Kostyuk; Olga Gurina; Vladimir Chekhonin Journal: Int J Mol Sci Date: 2022-01-22 Impact factor: 5.923