E Raposio1, S Bonomini2, F Calderazzi3. 1. Department of surgical sciences, plastic surgery section, university of Parma, via Gramsci 14, 43100 Parma, Italy. Electronic address: edoardo.raposio@unipr.it. 2. Department of clinical and experimental medicine, division of hematology, Parma university hospital, via Gramsci 14, 43100 Parma, Italy. Electronic address: sBonomimi@ao.pr.it. 3. Department of surgical sciences, orthopedic clinic, Parma university hospital, via Gramsci 14, 43100 Parma, Italy. Electronic address: filippo.calderazzi@icloud.com.
Abstract
INTRODUCTION: Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells. METHODS: This paper describes the procedure to obtain a 5-cm3 saline sample, containing the adipose-derived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction. RESULTS: A mean of 2.5×106 cells is isolated for each procedure; 35% (875000) of these are CD34+/CD45- cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLAABC) and negative (CD33, CD39, CD102, CD106, CD146, HLADR) cell-associated surface antigens, characterizing them as ASCs. CONCLUSIONS: This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures.
INTRODUCTION: Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells. METHODS: This paper describes the procedure to obtain a 5-cm3 saline sample, containing the adipose-derived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction. RESULTS: A mean of 2.5×106 cells is isolated for each procedure; 35% (875000) of these are CD34+/CD45- cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLAABC) and negative (CD33, CD39, CD102, CD106, CD146, HLADR) cell-associated surface antigens, characterizing them as ASCs. CONCLUSIONS: This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures.
Authors: Marco Gardani; Nicolò Bertozzi; Michele Pio Grieco; Marianna Pesce; Francesco Simonacci; PierLuigi Santi; Edoardo Raposio Journal: Ann Med Surg (Lond) Date: 2017-07-20
Authors: P Netsch; S Elvers-Hornung; S Uhlig; H Klüter; V Huck; F Kirschhöfer; G Brenner-Weiß; K Janetzko; H Solz; P Wuchter; P Bugert; K Bieback Journal: Stem Cell Res Ther Date: 2018-07-04 Impact factor: 6.832
Authors: Jeremy V Lynn; Kavitha Ranganathan; Kevin M Urlaub; Alexandra O Luby; Chris J Stephan; Alexis Donneys; Noah S Nelson; Steven R Buchman Journal: Ann Plast Surg Date: 2020-07 Impact factor: 1.763