Ann E Rozner1, Maureen Durning2,3, Jenna Kropp1, Gregory J Wiepz2, Thaddeus G Golos4,5,6. 1. Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, USA. 2. Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA. 3. Obstetrics and Gynecology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA. 4. Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, USA. golos@primate.wisc.edu. 5. Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA. golos@primate.wisc.edu. 6. Obstetrics and Gynecology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA. golos@primate.wisc.edu.
Abstract
PROBLEM: Immune cells within the endometrium at implantation are thought to play an important role in implantation, although their exact role is not well understood. METHOD OF STUDY: A co-culture system of rhesus monkey embryos and maternal immune cells was established. Blastocysts obtained by in vitro fertilization were co-cultured with peripheral blood cells or decidual macrophages. Culture media were collected to assess secretions. Embryo growth was monitored, and trophoblasts were evaluated for proliferation, apoptosis, and differentiation. RESULTS: Embryonic trophoblast outgrowths were visible within 6 days of culture, and the area of embryo outgrowth was reduced when blastocysts were cultured with peripheral-derived or decidual macrophages. Trophoblast proliferation was not significantly affected with macrophage co-culture while chorionic gonadotropin secretion was increased. Trophoblast expression of CDH 11 and GJA1 was increased, suggesting that macrophages accelerate differentiation of peri-implantation trophoblasts. CONCLUSIONS: These results indicate an important role of macrophages in placentation and pregnancy success.
PROBLEM: Immune cells within the endometrium at implantation are thought to play an important role in implantation, although their exact role is not well understood. METHOD OF STUDY: A co-culture system of rhesus monkey embryos and maternal immune cells was established. Blastocysts obtained by in vitro fertilization were co-cultured with peripheral blood cells or decidual macrophages. Culture media were collected to assess secretions. Embryo growth was monitored, and trophoblasts were evaluated for proliferation, apoptosis, and differentiation. RESULTS: Embryonic trophoblast outgrowths were visible within 6 days of culture, and the area of embryo outgrowth was reduced when blastocysts were cultured with peripheral-derived or decidual macrophages. Trophoblast proliferation was not significantly affected with macrophage co-culture while chorionic gonadotropin secretion was increased. Trophoblast expression of CDH 11 and GJA1 was increased, suggesting that macrophages accelerate differentiation of peri-implantation trophoblasts. CONCLUSIONS: These results indicate an important role of macrophages in placentation and pregnancy success.
Authors: Ann E Rozner; Svetlana V Dambaeva; Jessica G Drenzek; Maureen Durning; Thaddeus G Golos Journal: Am J Reprod Immunol Date: 2011-01-31 Impact factor: 3.886
Authors: Thaddeus G Golos; Gennadiy I Bondarenko; Edith E Breburda; Svetlana V Dambaeva; Maureen Durning; Igor I Slukvin Journal: Methods Mol Med Date: 2006
Authors: Lindsey N Block; Matthew T Aliota; Thomas C Friedrich; Michele L Schotzko; Katherine D Mean; Gregory J Wiepz; Thaddeus G Golos; Jenna Kropp Schmidt Journal: Biol Reprod Date: 2020-04-15 Impact factor: 4.285
Authors: T Arthur Chang; Gennadiy I Bondarenko; Behzad Gerami-Naini; Jessica G Drenzek; Maureen Durning; Mark A Garthwaite; Jenna Kropp Schmidt; Thaddeus G Golos Journal: Reprod Biol Endocrinol Date: 2018-03-16 Impact factor: 5.211
Authors: Jenna Kropp Schmidt; Logan T Keding; Lindsey N Block; Gregory J Wiepz; Michelle R Koenig; Michael G Meyer; Brittany M Dusek; Kamryn M Kroner; Mario J Bertogliat; Avery R Kallio; Katherine D Mean; Thaddeus G Golos Journal: Sci Rep Date: 2020-11-05 Impact factor: 4.379