| Literature DB >> 27637148 |
Dorine Sichien1, Charlotte L Scott1, Liesbet Martens2, Matthias Vanderkerken3, Sofie Van Gassen4, Maud Plantinga3, Thorsten Joeris5, Sofie De Prijck1, Leen Vanhoutte6, Manon Vanheerswynghels3, Gert Van Isterdael3, Wendy Toussaint3, Filipe Branco Madeira3, Karl Vergote3, William W Agace7, Björn E Clausen8, Hamida Hammad3, Marc Dalod9, Yvan Saeys10, Bart N Lambrecht11, Martin Guilliams12.
Abstract
Interferon regulatory factor-8 (IRF8) has been proposed to be essential for development of monocytes, plasmacytoid dendritic cells (pDCs) and type 1 conventional dendritic cells (cDC1s) and remains highly expressed in differentiated DCs. Transcription factors that are required to maintain the identity of terminally differentiated cells are designated "terminal selectors." Using BM chimeras, conditional Irf8(fl/fl) mice and various promotors to target Cre recombinase to different stages of monocyte and DC development, we have identified IRF8 as a terminal selector of the cDC1 lineage controlling survival. In monocytes, IRF8 was necessary during early but not late development. Complete or late deletion of IRF8 had no effect on pDC development or survival but altered their phenotype and gene-expression profile leading to increased T cell stimulatory function but decreased type 1 interferon production. Thus, IRF8 differentially controls the survival and function of terminally differentiated monocytes, cDC1s, and pDCs.Entities:
Keywords: IRF4; IRF8; dendritic cell; development; lineage; monocyte; plasmacytoid dendritic cell; terminal selector; transcription factor
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Year: 2016 PMID： 27637148 DOI： 10.1016/j.immuni.2016.08.013
Source DB: PubMed Journal: Immunity ISSN： 1074-7613 Impact factor: 31.745