| Literature DB >> 27634645 |
Omer Salman Qureshi1, Alam Zeb1, Muhammad Akram1, Myung-Sic Kim1, Jong-Ho Kang1, Hoo-Seong Kim1, Arshad Majid2, Inbo Han3, Sun-Young Chang4, Ok-Nam Bae5, Jin-Ki Kim6.
Abstract
The aim of this study was to enhance the anti-inflammatory effects of carbon monoxide (CO) via sustained release of CO from carbon monoxide-releasing molecule-2-loaded lipid nanoparticles (CORM-2-NPs). CORM-2-NPs were prepared by hot high pressure homogenization method using trilaurin as a solid lipid core and Tween 20/Span 20/Myrj S40 as surfactant mixture. The physicochemical properties of CORM-2-NPs were characterized and CO release from CORM-2-NPs was assessed by myoglobin assay. In vitro anti-inflammatory effects were evaluated by nitric oxide assay in lipopolysaccharide-stimulated RAW 264.7 macrophages. In vivo anti-inflammatory activity was investigated by measuring paw volumes and histological examination in carrageenan-induced rat paw edema. Spherical CORM-2-NPs were around 100nm with narrow particle size distribution. The sustained CO release from CORM-2-NPs was observed and the half-life of CO release increased up to 10 times compared with CORM-2 solution. CORM-2-NPs showed enhanced in vitro anti-inflammatory effects by inhibition of nitric oxide production. Edema volume in rat paw was significantly reduced after treatment with CORM-2-NPs. Taken together, CORM-2-NPs have a great potential for CO therapeutics against inflammation via sustained release of CO.Entities:
Keywords: Anti-inflammatory effect; CORM-2; Carbon monoxide; Lipid nanoparticles; Sodium dithionite (PubChem CID: 24489); Sustained release; Tricarbonyldichlororuthenium(II) dimer (PubChem CID: 10951331); Trilaurin (PubChem CID: 10851); Tween 20 (PubChem CID: 443314)
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Year: 2016 PMID： 27634645 DOI： 10.1016/j.ejpb.2016.09.008
Source DB: PubMed Journal: Eur J Pharm Biopharm ISSN： 0939-6411 Impact factor: 5.571