| Literature DB >> 27631429 |
Rie Tanaka1,2, Risa Inagaki1, Naoki Sugimoto2, Hiroshi Akiyama2, Akito Nagatsu3.
Abstract
A quantitative 1H-NMR method (1H-qNMR) was developed to determine the concentration of acteoside and geniposidic acid in Plantaginis semen, which is an important crude drug for diuretic purposes. The purity of geniposidic acid and acteoside was determined by the ratio of the intensity of the H-3 signal at δ 7.51 ppm or the H-7″ signal at δ 7.58 ppm in methanol-d 4 to that of a hexamethyldisilane (HMD) signal at 0.04 ppm, respectively. The concentration of HMD was corrected with the International System of Units traceability using potassium hydrogen phthalate of certified reference material grade. The geniposidic acid content in two batches of Plantaginis semen as determined by 1H-qNMR was found to be 0.84 and 1.00 %, and the acteoside content was determined to be 0.80 and 0.93 %. We demonstrated that this method is useful for the quantitative analysis of geniposidic acid and acteoside in Plantainis semen.Entities:
Keywords: 1H-qNMR; Acteoside; Geniposidic acid; Plantaginis semen; Reagent purity
Mesh：
Substances：
Year: 2016 PMID： 27631429 DOI： 10.1007/s11418-016-1040-y
Source DB: PubMed Journal: J Nat Med ISSN： 1340-3443 Impact factor: 2.343