S Vidyalakshmi1, R Madhavan Nirmal2, V Veeravarmal2, A Santhadevy3, R Aravindhan4. 1. Senior Lecturer, Department of Oral and Maxillofacial Pathology, Indira Gandhi Institute of Dental Sciences , Pillaiyarkuppam, Pondicherry, India . 2. Professor, Department of Oral and Maxillofacial Pathology, Rajah Muthiah Dental College and Hospital, Annamalai University , Chidambaram, India . 3. Professor, Department of Oral and Maxillofacial Pathology, Indira Gandhi Institute of Dental Sciences , Pillaiyarkuppam, Pondicherry, India . 4. Senior Lecturer, Department of Oral and Maxillofacial Pathology, SRM Kattankulathur Dental College & Hospital, SRM University , Potheri, Kanchipuram dt, India .
Abstract
INTRODUCTION: The malignant transformation rate of Oral Lichen Planus (OLP) is between 0% and 5.8%. Oral lesions of lichen planus clinically presents itself multifocally, simulating the process of field cancerization in high risk malignancies. The Buccal MicroNucleus Cytome Assay (BMN Assay) provides a platform to identify the high risk individuals by evaluating the markers of nuclear damage at an earliest micro invasive phase. AIM: To evaluate DNA damage in exfoliated buccal mucosal cells in individuals with oral lichen planus lesions and thereby to delineate the high risk group. MATERIALS AND METHODS: Buccal smears from 22 OLP and 10 control samples were stained in modified Feulgen-Rossenback reaction for micronuclei assay. Cytological evaluation of number of MicroNucleated cells (CMN), Total Number of Micronuclei (TMN) in micronucleated cells was done in both groups. RESULTS: Frequency of micronucleated cells (CMN) when compared among the study and control group, a mean value of 4.27 ± 1.80 and 0.90 ± 0.88 were obtained respectively. On comparing the total number of micronuclei in the micronucleated cells (TMN) between the study and control groups, a mean value of 5.38 ± 2.42 and 1.5 ± 0.88 were obtained respectively. CONCLUSION: There was a significant increase in the frequency of micronuclei and the micronucleated cells in the oral lichen planus as compared to normal individuals.
INTRODUCTION: The malignant transformation rate of Oral Lichen Planus (OLP) is between 0% and 5.8%. Oral lesions of lichen planus clinically presents itself multifocally, simulating the process of field cancerization in high risk malignancies. The Buccal MicroNucleus Cytome Assay (BMN Assay) provides a platform to identify the high risk individuals by evaluating the markers of nuclear damage at an earliest micro invasive phase. AIM: To evaluate DNA damage in exfoliated buccal mucosal cells in individuals with oral lichen planus lesions and thereby to delineate the high risk group. MATERIALS AND METHODS: Buccal smears from 22 OLP and 10 control samples were stained in modified Feulgen-Rossenback reaction for micronuclei assay. Cytological evaluation of number of MicroNucleated cells (CMN), Total Number of Micronuclei (TMN) in micronucleated cells was done in both groups. RESULTS: Frequency of micronucleated cells (CMN) when compared among the study and control group, a mean value of 4.27 ± 1.80 and 0.90 ± 0.88 were obtained respectively. On comparing the total number of micronuclei in the micronucleated cells (TMN) between the study and control groups, a mean value of 5.38 ± 2.42 and 1.5 ± 0.88 were obtained respectively. CONCLUSION: There was a significant increase in the frequency of micronuclei and the micronucleated cells in the oral lichen planus as compared to normal individuals.
Authors: G Casartelli; S Bonatti; M De Ferrari; M Scala; P Mereu; G Margarino; A Abbondandolo Journal: Anal Quant Cytol Histol Date: 2000-12 Impact factor: 0.302
Authors: Khalil Assiri; Mohammad Shahul Hameed; Ali Azhar Dawasaz; Eman Alamoudi; Alhussain Mohammad Asiri; Vij Hitesh; Muhammed Ajmal Journal: Indian J Dermatol Date: 2020 Jul-Aug Impact factor: 1.494