N Schlabritz-Loutsevitch1, K Apostolakis-Kyrus2, R Krutilina3, G Hubbard4, M Kocak5, Z Janjetovic6, S Sathanandam7, A T Slominski6,8, G Mari2, E Dick9. 1. Department of Obstetrics and Gynecology, Texas Tech University Health Sciences Center at the Permian Basin, Odessa, TX, USA. 2. Department of Obstetrics and Gynecology, University of Tennessee Health Science Center, Memphis, TN, USA. 3. Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, TN, USA. 4. Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA. 5. Division of Biostatistics and Epidemiology, Department of Preventive Medicine, University of Tennessee Health Science Center, Memphis, TN, USA. 6. Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA. 7. Division of Pediatric Cardiology, University of Tennessee Health Science Center, Memphis, TN, USA. 8. VA Medical Center, Birmingham AL, USA. 9. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA.
Abstract
BACKGROUND: Obesity in pregnancy (MO) is a risk factor for maternal and/or fetal cardiovascular system disorders. This study evaluated maternal CVS expression of microRNA-29 family and its target molecules in MO to test the hypotheses: CVS miR-29 concentrations are increased in pregnancy and decreased in MO. METHODS: Non-pregnant (n=4), pregnant obese (POb, n=4), and pregnant non-obese (PnOb, n=4) baboons (Papio spp.) were studied. Maternal left ventricle (LV), left atrium (LA), and aortic arch (AA) were collected at the end of gestation. Expression of MiR-29 and elastin (ELN) mRNA were quantified. RESULTS: LA miR-29 (a, c) expression was highest in PnOb. In the LV, miR-29b expression trended lower (P=.059) for PnOb animals. ELN mRNA expression correlated positively with miR-29b expression in AA (r=.76, P=.03). CONCLUSION: Maternal obesity diminishes miR-29 adaptation to pregnancy. Pharmacologic, tissue-specific targeting of miRNA-29 may represent a strategy for prevention and treatment of MO complications.
BACKGROUND:Obesity in pregnancy (MO) is a risk factor for maternal and/or fetal cardiovascular system disorders. This study evaluated maternal CVS expression of microRNA-29 family and its target molecules in MO to test the hypotheses: CVS miR-29 concentrations are increased in pregnancy and decreased in MO. METHODS: Non-pregnant (n=4), pregnant obese (POb, n=4), and pregnant non-obese (PnOb, n=4) baboons (Papio spp.) were studied. Maternal left ventricle (LV), left atrium (LA), and aortic arch (AA) were collected at the end of gestation. Expression of MiR-29 and elastin (ELN) mRNA were quantified. RESULTS: LA miR-29 (a, c) expression was highest in PnOb. In the LV, miR-29b expression trended lower (P=.059) for PnOb animals. ELN mRNA expression correlated positively with miR-29b expression in AA (r=.76, P=.03). CONCLUSION:Maternal obesity diminishes miR-29 adaptation to pregnancy. Pharmacologic, tissue-specific targeting of miRNA-29 may represent a strategy for prevention and treatment of MO complications.
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