Yuan Yue1,2, Xingmei Yang3,4, Liang Zhang1,5, Xun Xiao1,2, Neel R Nabar6, Yunfeng Lin1, Liang Hao1, Dongjiao Zhang1,2, Jingyi Huo1,2, Jingle Li1,2, Xiaoxiao Cai1,5, Min Wang7,8. 1. State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China. 2. Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu, China. 3. State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China. 65167950@qq.com. 4. Oral Implant Center, West China School of Stomatology, Sichuan University, Chengdu, China. 65167950@qq.com. 5. Oral Implant Center, West China School of Stomatology, Sichuan University, Chengdu, China. 6. Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA. 7. State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China. hxkqwangm@163.com. 8. Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu, China. hxkqwangm@163.com.
Abstract
OBJECTIVES: Peripheral nerve injuries are a common occurrence, resulting in considerable patient suffering; it also represents a major economic burden on society. To improve treatment options following peripheral nerve injuries, scientists aim to find a way to promote Schwann cell (SC) myelination to help nerves to carry out their functions effectively. In this study, we investigated myelination ability of SCs, regulated by co-culture with adipose-derived stem cells (ASCs) or low-intensity pulsed ultrasound (LIPUS), and synergistic effects of combined treatments. MATERIALS AND METHODS: Schwann cells were co-cultured with or without ASCs, and either left untreated or treated with LIPUS for 10 min/d for 1, 4 or 7 days. Effects of LIPUS and ASC co-culture on pro-myelination indicators of SCs were analysed by real-time PCR (RT-PCR), Western blotting and immunofluorescence staining (IF). RESULTS: Our results indicate that ASC-SC co-culture and LIPUS, together or individually, promoted mRNA levels of epidermal growth factor receptor 3 (EGFR3/ErbB3), neuregulin1 (NRG1), early growth response protein 2 (Egr2/Krox20) and myelin basic protein (MBP), with corresponding increases in protein levels of ErbB3, NRG1 and Krox20. Interestingly, combination of ASC-SC co-culture and LIPUS displayed the most remarkable effects. CONCLUSION: We demonstrated that ASCs upregulated pro-myelination indicators of SCs by indirect contact (through co-culture) and that effects could be potentiated by LIPUS. We conclude that LIPUS, as a mechanical stress, may have potential in nerve regeneration with potential clinical relevance.
OBJECTIVES: Peripheral nerve injuries are a common occurrence, resulting in considerable patient suffering; it also represents a major economic burden on society. To improve treatment options following peripheral nerve injuries, scientists aim to find a way to promote Schwann cell (SC) myelination to help nerves to carry out their functions effectively. In this study, we investigated myelination ability of SCs, regulated by co-culture with adipose-derived stem cells (ASCs) or low-intensity pulsed ultrasound (LIPUS), and synergistic effects of combined treatments. MATERIALS AND METHODS: Schwann cells were co-cultured with or without ASCs, and either left untreated or treated with LIPUS for 10 min/d for 1, 4 or 7 days. Effects of LIPUS and ASC co-culture on pro-myelination indicators of SCs were analysed by real-time PCR (RT-PCR), Western blotting and immunofluorescence staining (IF). RESULTS: Our results indicate that ASC-SC co-culture and LIPUS, together or individually, promoted mRNA levels of epidermal growth factor receptor 3 (EGFR3/ErbB3), neuregulin1 (NRG1), early growth response protein 2 (Egr2/Krox20) and myelin basic protein (MBP), with corresponding increases in protein levels of ErbB3, NRG1 and Krox20. Interestingly, combination of ASC-SC co-culture and LIPUS displayed the most remarkable effects. CONCLUSION: We demonstrated that ASCs upregulated pro-myelination indicators of SCs by indirect contact (through co-culture) and that effects could be potentiated by LIPUS. We conclude that LIPUS, as a mechanical stress, may have potential in nerve regeneration with potential clinical relevance.
Authors: Sang Chul Park; Se Heang Oh; Tae Beom Seo; Uk Namgung; Jin Man Kim; Jin Ho Lee Journal: J Biomed Mater Res B Appl Biomater Date: 2010-08 Impact factor: 3.368