Gang Shao1, Michael MacNeil2, Yuanyuan Yao1, Roger W Giese1. 1. Department of Pharmaceutical Sciences and Barnett Institute, Bouve College, Northeastern University, Boston, MA, 02115, USA. 2. Department of Engineering, College of Engineering, Northeastern University, Boston, MA, 02115, USA.
Abstract
RATIONALE: A method was needed to accomplish solid-phase extraction of a large urine volume in a convenient way where resources are limited, towards a goal of metabolome and xenobiotic exposome analysis at another, distant location. METHODS: A porous extraction paddle (PEP) was set up, comprising a porous nylon bag containing extraction particles that is flattened and immobilized between two stainless steel meshes. Stirring the PEP after attachment to a shaft of a motor mounted on the lid of the jar containing the urine accomplishes extraction. The bag contained a mixture of nonpolar and partly nonpolar particles to extract a diversity of corresponding compounds. RESULTS: Elution of a urine-exposed, water-washed PEP with aqueous methanol containing triethylammonium acetate (conditions intended to give a complete elution), followed by MALDI-TOF/TOF-MS, demonstrated that a diversity of compounds had been extracted ranging from uric acid to peptides. CONCLUSIONS: The PEP allows the user to extract a large liquid sample in a jar simply by turning on a motor. The technique will be helpful in conducting metabolomics and xenobiotic exposome studies of urine, encouraging the extraction of large volumes to set up a convenient repository sample (e.g. 2 g of exposed adsorbent in a cryovial) for shipment and re-analysis in various ways in the future, including scaled-up isolation of unknown chemicals for identification.
RATIONALE: A method was needed to accomplish solid-phase extraction of a large urine volume in a convenient way where resources are limited, towards a goal of metabolome and xenobiotic exposome analysis at another, distant location. METHODS: A porous extraction paddle (PEP) was set up, comprising a porous nylon bag containing extraction particles that is flattened and immobilized between two stainless steel meshes. Stirring the PEP after attachment to a shaft of a motor mounted on the lid of the jar containing the urine accomplishes extraction. The bag contained a mixture of nonpolar and partly nonpolar particles to extract a diversity of corresponding compounds. RESULTS: Elution of a urine-exposed, water-washed PEP with aqueous methanol containing triethylammonium acetate (conditions intended to give a complete elution), followed by MALDI-TOF/TOF-MS, demonstrated that a diversity of compounds had been extracted ranging from uric acid to peptides. CONCLUSIONS: The PEP allows the user to extract a large liquid sample in a jar simply by turning on a motor. The technique will be helpful in conducting metabolomics and xenobiotic exposome studies of urine, encouraging the extraction of large volumes to set up a convenient repository sample (e.g. 2 g of exposed adsorbent in a cryovial) for shipment and re-analysis in various ways in the future, including scaled-up isolation of unknown chemicals for identification.
Authors: Yuanyuan Yao; Poguang Wang; Gang Shao; Liza V Anzalota Del Toro; Jose Codero; Roger W Giese Journal: Rapid Commun Mass Spectrom Date: 2016-11-15 Impact factor: 2.419
Authors: Yishan Lin; Maria Sevillano-Rivera; Tao Jiang; Guangyu Li; Irmarie Cotto; Solize Vosloo; Corey M G Carpenter; Philip Larese-Casanova; Roger W Giese; Damian E Helbling; Ingrid Y Padilla; Zaira Rosario-Pabón; Carmen Vélez Vega; José F Cordero; Akram N Alshawabkeh; Ameet Pinto; April Z Gu Journal: Environ Sci Technol Date: 2020-07-23 Impact factor: 9.028
Authors: Yuanyuan Yao; Poguang Wang; Gang Shao; Liza V Anzalota Del Toro; Jose Codero; Roger W Giese Journal: Rapid Commun Mass Spectrom Date: 2016-11-15 Impact factor: 2.419