Anastasia Spyrogianni1, Inge K Herrmann2, Miriam S Lucas3, Jean-Christophe Leroux4, Georgios A Sotiriou4,5. 1. Particle Technology Laboratory, Institute of Process Engineering, Department of Mechanical & Process Engineering, ETH Zurich, Sonneggstrasse 3, CH-8092 Zurich, Switzerland. 2. Particles-Biology Interactions Laboratory, Department of Materials Meet Life, Swiss Federal Laboratories for Materials Science & Technology (Empa), Lerchenfeldstrasse 5, CH-9014 St Gallen, Switzerland. 3. Scientific Center for Optical & Electron Microscopy (ScopeM), ETH Zurich, Auguste-Piccard-Hof 1, CH-8093 Zurich, Switzerland. 4. Drug Formulation & Delivery, Institute of Pharmaceutical Sciences, Department of Chemistry & Applied Biosciences, ETH Zurich, Vladimir-Prelog-Weg 3, CH-8093 Zurich, Switzerland. 5. Department of Microbiology, Tumor & Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden.
Abstract
AIM: The delivered nanoparticle dose to cells in vitro may depend on nanoparticle sedimentation rate. Here, the conditions under which optical absorption spectroscopy can be used to quantify the deposited nanoparticle dose in vitro are investigated. MATERIALS & METHODS: Nanoparticle cytotoxicity in both upright and inverted cell culture orientations is studied in the presence and absence of serum. RESULTS: Dissolvable nanoparticles, such as ZnO, exhibit no difference in upright and inverted cultures due to dissolved Zn(2+) ions that dominate cytotoxicity. Insoluble nanoparticles, however, exhibit different sedimentation rates and deposited doses that are linked to the observed cytotoxicity. CONCLUSION: The combined use of upright-inverted cell orientations and optical absorption spectroscopy can provide a simple experimental approach to interpret in vitro nano-biointeractions.
AIM: The delivered nanoparticle dose to cells in vitro may depend on nanoparticle sedimentation rate. Here, the conditions under which optical absorption spectroscopy can be used to quantify the deposited nanoparticle dose in vitro are investigated. MATERIALS & METHODS: Nanoparticle cytotoxicity in both upright and inverted cell culture orientations is studied in the presence and absence of serum. RESULTS: Dissolvable nanoparticles, such as ZnO, exhibit no difference in upright and inverted cultures due to dissolved Zn(2+) ions that dominate cytotoxicity. Insoluble nanoparticles, however, exhibit different sedimentation rates and deposited doses that are linked to the observed cytotoxicity. CONCLUSION: The combined use of upright-inverted cell orientations and optical absorption spectroscopy can provide a simple experimental approach to interpret in vitro nano-biointeractions.
Authors: Aleksandra Zielińska; Beatriz Costa; Maria V Ferreira; Diogo Miguéis; Jéssica M S Louros; Alessandra Durazzo; Massimo Lucarini; Piotr Eder; Marco V Chaud; Margreet Morsink; Niels Willemen; Patrícia Severino; Antonello Santini; Eliana B Souto Journal: Int J Environ Res Public Health Date: 2020-06-28 Impact factor: 3.390