Olfactory functioning in early multiple sclerosis: Sniffin' Sticks Test study.

INTRODUCTION: Previous studies have shown that olfactory functioning is affected by multiple sclerosis (MS). This study assessed the level of the olfactory impairment in early MS by using the Sniffin' Sticks Test.
METHODS: This study included 30 patients with MS and 30 healthy controls. We collected demographic and clinical data from participants and administered the Sniffin' Sticks Test.
RESULTS: We found no differences between the MS and control groups in odor discrimination, odor identification, and threshold discrimination identification scores, but odor threshold (OT) scores were higher in the control group than in the MS group (P=0.49). In addition, we did not find any correlation between MS patients' olfactory test scores and their scores on the Mini-Mental State Examination (MMSE), Expanded Disability Status Scale (EDSS), disease duration, history of optic neuritis, or being on immunomodulatory therapy.
CONCLUSION: In recent studies, odor threshold impairment seemed to be the most striking finding in patients with MS. Although the present study found a mild alteration in odor threshold, olfactory dysfunction appears to be a consequence of neurodegeneration in the higher order olfactory brain regions, which is thought to be a time-dependent process.

Introduction

The olfactory function is generated by chemical signals in the peripheral olfactory system and is important to human survival and perception.1 Olfaction has been extensively studied in the context of neurological diseases such as Parkinson’s disease,1,2 Alzheimer’s disease,3 multiple sclerosis (MS),4–9 and migraine.10 In patients with MS, olfactory dysfunction is associated with demyelination and the early axonal degeneration of olfactory brain regions, namely the inferior frontal and temporal lobes.4

Since the 1990s, several olfactory test protocols have been used. The most common ones are: the University of Pennsylvania Smell Identification Test, the Connecticut Chemosensory Clinical Research Center Test, and the Sniffin’ Sticks Test.11 The Sniffin’ Sticks Test is available for olfactory assessment in Turkey, Europe, and Australia.11 It is a well-designed olfactory test that the German Olfactology and Gustology Association12 recommends and uses in many studies that assess the olfactory functioning of MS patients.

The Sniffin’ Sticks Test consists of a set of three olfactory tests that require the use of pen-like odor-dispensing devices. These three tests result in odor identification (OI), odor discrimination (OD), and odor threshold (OT) scores, which were recorded for this study. The sum of the three subtests is presented as a composite threshold discrimination identification (TDI) score. Normative data for olfaction using the Sniffin’ Sticks test have been published in Turkey, but none of these studies include patients with MS.11

The present study assesses the olfactory impairment of patients with MS according to the duration of the disease, the patients’ smoking habits, and their scores on the Mini–Mental State Examination (MMSE) and the Expanded Disability Status Scale (EDSS).

Methods

This study includes 30 healthy volunteers and 30 patients with MS (according to the McDonald Revised Criteria, 2005),13 who were evaluated in the Neurology Department of the Gazi University Faculty of Medicine. The study was carried out in accordance with the Declaration of Helsinki and approved by the Faculty of Medicine’s ethics committee at Gazi University. All volunteers were at least 18 years of age and provided written informed consent prior to participating in this study. We recorded the participants’ age, sex, and smoking habits. If participants had complaints about odor sensitivity, nasal surgery, head trauma, nasal polyps, rhinosinusitis, an upper respiratory tract infection, or about or any known olfactory diseases, then they were excluded from this study. The control group consisted of healthy individuals without neurological diseases or olfactory system abnormalities.

Clinical data were collected from MS patients and a neurological examination was administered. Patients who experienced an MS attack and who took steroids in the month prior to the study were also excluded. In addition, we also collected the following forms of data: the time since the patient’s MS diagnosis, their history of optic neuritis, the medical therapy that they received for MS, as well as data from the EDSS and MMSE.

After providing demographic information and undergoing a clinical assessment, patients with MS and healthy controls performed the Sniffin’ Sticks Test to evaluate their olfactory functioning. This test (Heinrich Burghart, GmbH, Wedel, Germany)14 includes three subtests that measure OT, OD, and OI. The scores of OT, OD, and OI were recorded. Finally the sum of the three test scores was calculated to provide the composite TDI score.

Statistical analyses

There was a 90% power of detecting true OT, OD, and OI score differences in the composite TDI scores when a minimum of 25 patients were included in each group, resulting in a 5% significance level.6 Statistical analyses were performed using the SPSS 15.0 software package (SPSS Inc., Chicago, IL, USA). Both t-tests and chi-squared tests were used to compare the demographic and clinical characteristics of the control and the MS groups. Within the MS group, a Pearson’s correlation analysis assessed the relationship between the MS patients’ clinical characteristics and their olfactory functioning. Two-way analysis of variance then examined the effects of smoking, headaches, and sex on each group. Values of P<0.05 were considered as statistically significant.

Results

Demographic and clinical characteristics

This study included 27 patients with relapsing remitting multiple sclerosis, three patients with secondary progressive multiple sclerosis, and 30 healthy controls. McDonald’s Revised Criteria13 were used to diagnose MS. Demographic and clinical characteristics of the study participants are summarized in Table 1. The age of MS patients’ ranged from 18 to 49 years (mean 34.3, standard deviation ±9.8), and the healthy controls were matched for age and smoking habits (Table 1).

Table 1

Demographic and clinical characteristics of MS and control groups

Characteristics	MS	Control	P-value
Age in years: mean, ± SD (min–max)	34.3, ±9.8 (18–49)	35.8, ±9.2 (19–50)	0.526
Sex: n (% female)	21 (70%)	21 (70%)	0.241
Current smoking status: n (% smokers)	10 (33.3%)	9 (30%)	0.278
MMSE: mean, ± SD	29.03, ±2.22	29.97, ±0.18	0.025*
Disease duration in months: mean, ± SD	47.7, ±48
EDSS: mean, ± SD	1.91, ±1.57
Optic neuritis: n (%)	18 (60%)
Immunomodulatory therapy: n (%)	25 (83.3%)

Note:

P<0.05.

Abbreviations: EDSS, Expanded Disability Status Scale; max, maximum; min, minimum; MMSE, Mini–Mental State Examination; MS, multiple sclerosis; SD, standard deviation.

Olfactory functioning

The results of olfactory tests were analyzed in terms of OT, OD, OI, and TDI scores. While examining the OD, OI, and TDI scores, no differences between the MS and the control groups were found. However, the OT scores were higher in the control group than in the MS group (P=0.49) (Table 2).

Table 2

Olfactory test scores for MS and control groups

Scores	MS	Control	P-value
OT: mean, ± SD	11.04, ±3.28	12.55, ±2.51	0.049*
OD: mean, ± SD	10.83, ±2.30	11.37, ±2.31	0.375
OI: mean, ± SD	11.60, ±2.50	11.97, ±1.97	0.531
TDI: mean, ± SD	33.4, ±6.35	35.89, ±5.48	0.129

Note:

P<0.05.

Abbreviations: MS, multiple sclerosis; OD, odor discrimination; OI, odor identification; OT, odor threshold; SD, standard deviation; TDI, threshold discrimination identification.

Correlations between clinical characteristics and the results of olfactory tests

An application of Pearson’s correlation analysis revealed no correlation among demographic, clinical, and olfactory variables within the control group. Olfactory test scores of patients with MS were not associated with their MMSE scores, EDSS scores, disease duration, history of optic neuritis, or being on immunomodulatory therapy. However, the patients’ ages were associated with their OI, OT, and TDI scores; older patients had lower OI, OT, TDI scores (Table 3).

Table 3

Correlations between age, MMSE and EDSS scores, disease duration, and olfactory test scores for patients with MS

Olfactory test scores	Age		MMSE scores		EDSS scores		Disease duration
	r	P-value	r	P-value	r	P-value	r	P-value
OT	−0.365	0.047*	0.229	0.223	−0.286	0.125	−0.127	0.505
OD	−0.237	0.207	0.385	0.036	−0.142	0.454	−0.261	0.164
OI	−0.412	0.024*	0.450	0.013*	−0.321	0.084	−0.161	0.395
TDI	−0.436	0.016*	0.435	0.016*	−0.325	0.079	−0.223	0.235

Note:

P<0.05.

Abbreviations: EDSS, Expanded Disability Status Scale; MMSE, Mini–Mental State Examination; MS, multiple sclerosis; OD, odor discrimination; OI, odor identification; OT, odor threshold; TDI, threshold discrimination identification.

In addition, the correlations between disease duration and the olfactory test results were specifically evaluated. In order to assess whether there were significant differences between the Sniffin’ Sticks scores among these two groups, data from patients who had MS for ≥60 months were compared to that from patients who had MS for <60 months. There was no significant difference.

Discussion

Previously, it was assumed that the odor function was spared in patients with MS.15 More recent investigations that rely on advanced techniques for odor testing have demonstrated impairment in relation to various aspects of olfaction in patients with MS.8 The Sniffin’ Sticks Test is one of the most sophisticated ways of testing nasal chemosensory performance,16 and previous reports using this test have established normative data for many countries, including Turkey.11,12,17,18 Tekeli et al17 provided an update of normative values for Sniffin’ Sticks test in Turkish people and concluded that this test is applicable for clinical usage in Turkey without any cultural adaptation. The present study used the Sniffin’ Sticks test to evaluate olfactory functioning in patients with MS, but these results did not demonstrate hyposmia in patients with MS. Compared with MS patients enrolled in other studies,5,7,8 this sample was relatively young and in the earlier stages of the disease. In general, the patients enrolled in the present study did not exhibit cognitive decline and cooperated fully with the examiner. Their EDSS scores were lower than those in previous studies, and this might have affected the results of the study.5,7

Rolet et al7 observed that general damage to all three aspects of olfaction was found in 16% of patients. Primarily, these patients received natalizumab therapy and had more severe forms of MS when compared to the patients in this study. Furthermore, Silva et al5 found olfactory deficits in 68.8% of patients with secondary progressive multiple sclerosis but found no differences between those with relapsing remitting multiple sclerosis and a healthy control group. In this study, those in the later phases of MS did not demonstrate deterioration in their olfactory functioning. The patients who were diagnosed with MS at least 60 months prior to participating in this study had normal Sniffin’ Sticks scores. This may be due to a low density of demyelinative lesions and relative absence of neurodegeneration.

Despite the fact that primary olfactory neurons are unmyelinated, the olfactory processing regions like inferior frontal and temporal lobes are myelinated.4,19 Magnetic resonance imaging studies investigating the influence of demyelinating lesions have shown a negative correlation between odor identification test scores and lesion load in olfactory regions of the brain.4,9 Doty et al4 proposed that a sufficient number of plaques within olfactory eloquent regions of the brain give rise to olfactory dysfunction.

Lutterotti et al6 identified impaired OT and OI test results in MS patients with Sniffin’ Sticks Test. They found that the impairment of patients’ odor thresholds was more prominent in the early phases of MS, and they assumed that this was a result of clinical disease activity related to inflammation. Longer disease durations affected odor identification. Moreover, Rolet et al7 observed that 40% of patients with MS had odor thresholds that were the primarily affected aspect of olfactory function.

We can speculate that the progression of the disease and its acute inflammatory process influence the olfactory dysfunction. However, this study did not find any difference between patients with a disease duration of <60 months and those with disease duration of ≥60 months, indicating that olfaction is generally spared prior to the onset of neurodegeneration. This may be a result of the fact that the impairment in OT scores of MS patients is associated with the duration and the course of the disease. As patients with acute attacks in the previous month were excluded, this study could not determine whether the inflammatory phase initiated damage in the olfactory regions.

Olfactory loss is considered as a major consequence of aging and may be the early sign of neurodegenerative diseases. This could be the result of changes in olfactory bulb, degeneration of primary and secondary olfactory cortices.20 Olfactory dysfunction’s prevalence in dementia may be as high as 100% in Alzheimer’s disease, 90% in Parkinson disease, 96% in the frontal variant of frontotemporal dementia, and 15% in vascular dementia.21 MS-related olfactory loss is related to the number of plaques in olfactory regions (inferior frontal and temporal lobes)8 and may be a marker of disease progression. Zivadinov et al8 stated significant correlation between olfactory dysfunction and symptoms of anxiety, depression, and severity of neurological impairment.

Limitations

There are some limitations to our study. First, the group of participants was relatively small. Second, the study included mostly patients with relapsing remitting multiple sclerosis. The progressive courses of MS would lead to different levels of olfactory dysfunction. Third, EDSS scores were relatively low showing that the patients were mildly affected with MS. Fourth, the patients with acute attacks were excluded; hence, the effects of acute inflammatory process on olfactory function remained unknown. Fifth, magnetic resonance imaging data of patients were not included in the study. The presence of MS plaques in olfactory regions would have been enlightening. Further studies are needed to assess the correlation between plaque burden and olfactory dysfunction in MS. Sixth, the neurocognitive tests were not applied elaborately, only the MMSE was completed. The effects of cognitive deficits on olfactory functioning were not analyzed.

Conclusion

In recent studies, odor threshold impairment seemed to be the most striking finding in patients with MS. Although the present study found a mild alteration in OT, olfactory dysfunction appears to be a consequence of neurodegeneration in the higher-order olfactory brain regions, which is thought to be a time-dependent process.