Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Construction of a 42 base pair double stranded DNA microcircle.

Literature DB >> 2762124

Construction of a 42 base pair double stranded DNA microcircle.

Abstract

A procedure for the construction of double stranded DNA microcircles is described that overcomes the natural limits of established circularization procedures. Starting with two synthetic oligonucleotides which are able to form dumbbell shaped structures, two subsequent ligation reactions yield a microcircle of double stranded DNA of 42 base pairs. This is by far the smallest circle of double stranded DNA yet described. These microcircles can be constructed in quantities required for high resolution structural analyses such as X-ray crystallography and NMR spectroscopy.

Entities: Chemical

Mesh：

Substances：

Year: 1989 PMID： 2762124 PMCID： PMC318103 DOI： 10.1093/nar/17.13.5163

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

42 in total

1. Curved DNA: design, synthesis, and circularization.

Authors: L Ulanovsky; M Bodner; E N Trifonov; M Choder
Journal: Proc Natl Acad Sci U S A Date: 1986-02 Impact factor: 11.205

2. Multiple DNA-protein interactions governing high-precision DNA transactions.

Authors: H Echols
Journal: Science Date: 1986-09-05 Impact factor: 47.728

3. Topological distributions and the torsional rigidity of DNA. A Monte Carlo study of DNA circles.

Authors: S D Levene; D M Crothers
Journal: J Mol Biol Date: 1986-05-05 Impact factor: 5.469

4. Ring closure probabilities for DNA fragments by Monte Carlo simulation.

Authors: S D Levene; D M Crothers
Journal: J Mol Biol Date: 1986-05-05 Impact factor: 5.469

5. Visualization of the bent helix in kinetoplast DNA by electron microscopy.

Authors: J Griffith; M Bleyman; C A Rauch; P A Kitchin; P T Englund
Journal: Cell Date: 1986-08-29 Impact factor: 41.582

6. DNA loops induced by cooperative binding of lambda repressor.

Authors: J Griffith; A Hochschild; M Ptashne
Journal: Nature Date: 1986 Aug 21-27 Impact factor: 49.962

7. Three-dimensional structure of a DNA hairpin in solution: two-dimensional NMR studies and distance geometry calculations on d(CGCGTTTTCGCG).

Authors: D R Hare; B R Reid
Journal: Biochemistry Date: 1986-09-09 Impact factor: 3.162

3. Structural Landscape of the Transition from an ssDNA Dumbbell Plus Its Complementary Hairpin to a dsDNA Microcircle Via a Kissing Loop Intermediate.

Authors: Alberto Mills; Federico Gago
Journal: Molecules Date: 2021-05-19 Impact factor: 4.411

4. Rolling circle RNA synthesis catalyzed by RNA.

Authors: Emil Laust Kristoffersen; Matthew Burman; Agnes Noy; Philipp Holliger
Journal: Elife Date: 2022-02-02 Impact factor: 8.713

4 in total

Construction of a 42 base pair double stranded DNA microcircle.

1. Curved DNA: design, synthesis, and circularization.

2. Multiple DNA-protein interactions governing high-precision DNA transactions.

3. Topological distributions and the torsional rigidity of DNA. A Monte Carlo study of DNA circles.

4. Ring closure probabilities for DNA fragments by Monte Carlo simulation.

5. Visualization of the bent helix in kinetoplast DNA by electron microscopy.

6. DNA loops induced by cooperative binding of lambda repressor.

7. Three-dimensional structure of a DNA hairpin in solution: two-dimensional NMR studies and distance geometry calculations on d(CGCGTTTTCGCG).

8. DNA bending induced by the catabolite activator protein allows ring formation of a 144 bp DNA.

9. Structure of the DNA-Eco RI endonuclease recognition complex at 3 A resolution.

10. Diethyl pyrocarbonate: a chemical probe for secondary structure in negatively supercoiled DNA.

1. Recognition of DNA, RNA, and Proteins by Circular Oligonucleotides.

2. Reconstitution of uracil DNA glycosylase-initiated base excision repair in herpes simplex virus-1.

3. Structural Landscape of the Transition from an ssDNA Dumbbell Plus Its Complementary Hairpin to a dsDNA Microcircle Via a Kissing Loop Intermediate.

4. Rolling circle RNA synthesis catalyzed by RNA.