| Literature DB >> 27616694 |
Yin Wan1, Bin Zou2, Hailong Zeng2, Lunning Zhang3, Ming Chen4, Guiming Fu5.
Abstract
In this study, we analyzed the inhibitory effect of verbascoside against xanthine oxidase (XOD) in vitro by using animal model and in vivo by direct inhibition assay. Results showed that verbascoside could reduce uric acid in rat serum and inhibit XOD activity in rat liver. The IC50 value of restraining XOD activity was 81.11mgmL-1. Fluorescence chromatographic analysis and circular dichroism spectroscopy indicated that the secondary structures of XOD were changed after incubation with verbascoside. The docking simulation showed that verbascoside could enter into the active site of XOD and form hydrogen bonding with amino acid residues (such as Lys-1045, Arg-880, Arg-912, Glu-1261 and Gln-1194). The results suggested that verbascoside, which is a naturally occurring water-soluble antioxidant, could be a potential low-toxicity XOD inhibitor for hyperuricemia treatment.Entities:
Keywords: Circular dichroism spectra; Fluorescence chromatographic; Inhibitory activity; Molecular simulation; Verbascoside; Xanthine oxidase
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Year: 2016 PMID： 27616694 DOI： 10.1016/j.ijbiomac.2016.09.022
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953