| Literature DB >> 27616287 |
Md Hakimul Haque1, Vinod Gopalan2, Sharda Yadav3, Md Nazmul Islam3, Ehsan Eftekhari4, Qin Li4, Laura G Carrascosa5, Nam-Trung Nguyen6, Alfred K Lam7, Muhammad J A Shiddiky8.
Abstract
We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues. Copyright ÂEntities:
Keywords: DNA methylation; Disposable screen-printed electrode; Electrochemical detection; Graphene-DNA base interaction; Regional methylation
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Year: 2016 PMID： 27616287 DOI： 10.1016/j.bios.2016.09.016
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618