Daniela Paes Almeida Ferreira Braga1,2,3, Amanda Souza Setti1,3, Edson Guimarães Lo Turco2, Fernanda Bertuccez Cordeiro2, Elaine Cristina Cabral4, Sylvia Sanches Cortezzi3, Erika Ono3, Rita Cássia Sávio Figueira1, Marcos Nogueira Eberlin5, Edson Borges6,7. 1. Fertility Medical Group, Av. Brigadeiro Luis Antônio, 4545, São Paulo, SP, 01401-002, Brazil. 2. Disciplina de Urologia, Área de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo-UNIFESP, Rua Embaú, 231, São Paulo, SP, 04039-060, Brazil. 3. Instituto Sapientiae-Centro de Estudos e Pesquisa em Reprodução Humana Assistida, Rua Vieira Maciel, 62, São Paulo, SP, 04503-040, Brazil. 4. Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agronômicas (CPQBA)-Universidade de Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil. 5. Laboratório ThoMSon de Espectrometria de Massas-Instituto de Química, Universidade de Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil. 6. Fertility Medical Group, Av. Brigadeiro Luis Antônio, 4545, São Paulo, SP, 01401-002, Brazil. edson@fertility.com.br. 7. Disciplina de Urologia, Área de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo-UNIFESP, Rua Embaú, 231, São Paulo, SP, 04039-060, Brazil. edson@fertility.com.br.
Abstract
PURPOSE: The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). METHODS: Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. RESULTS: There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. CONCLUSIONS: CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.
PURPOSE: The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). METHODS: Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. RESULTS: There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. CONCLUSIONS: CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.
Entities:
Keywords:
Blastocyst; Cumulus cell; Mass spectrometry; Pregnancy; Proteomics
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