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Literature DB >> 2760933

Antibody against the amino terminus of alpha-actin inhibits actomyosin interactions in the presence of ATP.

Abstract

Actomyosin interactions in the presence of ATP were examined by using site-specific antibodies directed against the first seven N-terminal residues on skeletal alpha-actin. Fab fragments of these antibodies (S alpha N Fab) inhibited effectively the actin-activated ATPase of myosin subfragment 1 (S-1) at both 5 and 25 degrees C. Binding experiments carried out in the presence of ATP at 5 degrees C revealed that the catalytic inhibition was related to the inhibition of S-1 binding to actin by Fab. At equimolar ratios of Fab to actin, the binding of S-1 to actin and the activated ATPase were inhibited by 75 and 82%, respectively. These results, when contrasted with the small effect of Fab on rigor actomyosin binding, suggest ATP-induced changes at the interface of actin and myosin.

Entities: Chemical Gene

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Year: 1989 PMID： 2760933 DOI： 10.1016/0022-2836(89)90249-0

Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469

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Cited

21 in total

1. Influence of ionic strength on the actomyosin reaction steps in contracting skeletal muscle fibers.

Authors: H Iwamoto
Journal: Biophys J Date: 2000-06 Impact factor: 4.033

2. A mosaic multiple-binding model for the binding of caldesmon and myosin subfragment-1 to actin.

Authors: Y D Chen; J M Chalovich
Journal: Biophys J Date: 1992-10 Impact factor: 4.033

3. Role of the N-terminal negative charges of actin in force generation and cross-bridge kinetics in reconstituted bovine cardiac muscle fibres.

Authors: Xiaoying Lu; Mary K Bryant; Keith E Bryan; Peter A Rubenstein; Masataka Kawai
Journal: J Physiol Date: 2005-01-13 Impact factor: 5.182

10. Cross-bridge binding to actin and force generation in skinned fibers of the rabbit psoas muscle in the presence of antibody fragments against the N-terminus of actin.

Authors: B Brenner; T Kraft; G DasGupta; E Reisler
Journal: Biophys J Date: 1996-01 Impact factor: 4.033

Antibody against the amino terminus of alpha-actin inhibits actomyosin interactions in the presence of ATP.

1. Influence of ionic strength on the actomyosin reaction steps in contracting skeletal muscle fibers.

2. A mosaic multiple-binding model for the binding of caldesmon and myosin subfragment-1 to actin.

3. Role of the N-terminal negative charges of actin in force generation and cross-bridge kinetics in reconstituted bovine cardiac muscle fibres.

Review 4. Use of thin filament reconstituted muscle fibres to probe the mechanism of force generation.

5. Functional adaptation between yeast actin and its cognate myosin motors.

6. Site-directed mutations of Dictyostelium actin: disruption of a negative charge cluster at the N terminus.

7. Localization of a myosin subfragment-1 interaction site on the C-terminal part of actin.

8. Small segmental rearrangements in the myosin head can explain force generation in muscle.

9. Characterization of an actin-myosin head interface in the 40-113 region of actin using specific antibodies as probes.

10. Cross-bridge binding to actin and force generation in skinned fibers of the rabbit psoas muscle in the presence of antibody fragments against the N-terminus of actin.