Literature DB >> 27608539

Evaluation of a commercial immunochromatographic assay for rapid routine identification of PBP2a-positive Staphylococcus aureus and coagulase-negative staphylococci.

Céline Dupieux1, Sophie Trouillet-Assant2, Jason Tasse2, Anne-Marie Freydière3, Olivia Raulin3, Chantal Roure-Sobas3, Hélène Salord3, Sylvestre Tigaud3, Frédéric Laurent4.   

Abstract

We evaluated the performance of an immunochromatographic assay (PBP2a Culture Colony Test - Alere™), detecting protein-binding penicillin 2a on staphylococci primary isolates in only 6minutes. The assay is highly sensitive for the direct detection of MRSA on various culture media whereas it requires cefoxitin induction for methicillin-resistant coagulase-negative staphylococci.
Copyright © 2016 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Methicillin resistance; PBP2a detection; Rapid test; Staphylococci

Mesh:

Substances:

Year:  2016        PMID: 27608539     DOI: 10.1016/j.diagmicrobio.2016.08.012

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN: 0732-8893            Impact factor:   2.803


  5 in total

1.  Detection of mecC-Positive Staphylococcus aureus: What To Expect from Immunological Tests Targeting PBP2a?

Authors:  Céline Dupieux; Coralie Bouchiat; Anders Rhod Larsen; Bruno Pichon; Mark Holmes; Christopher Teale; Giles Edwards; Robert Hill; Jean-Winoc Decousser; Sophie Trouillet-Assant; Andreas Petersen; Robert Skov; Angela Kearns; Frédéric Laurent
Journal:  J Clin Microbiol       Date:  2017-03-15       Impact factor: 5.948

2.  Improved Performance of a Rapid Immunochromatographic Assay for Detection of PBP2a in Non-Staphylococcus aureus Staphylococcal Species.

Authors:  Matthew C Canver; Mark D Gonzalez; Bradley A Ford; Amanda R Arnold; Sara D Lawhon; C A Burnham; Stephen G Jenkins; Eileen M Burd; Lars F Westblade
Journal:  J Clin Microbiol       Date:  2019-03-28       Impact factor: 5.948

3.  Performance of the Revised Version of an Immunochromatographic Assay for Detection of mecA- and mecC-Mediated Methicillin Resistance in Staphylococci.

Authors:  Céline Dupieux; William Mouton; Camille André; François Vandenesch; Michèle Bes; Anne Tristan; Frédéric Laurent
Journal:  J Clin Microbiol       Date:  2019-12-23       Impact factor: 5.948

4.  Prevalence, Mechanism, Genetic Diversity, and Cross-Resistance Patterns of Methicillin-Resistant Staphylococcus Isolated from Companion Animal Clinical Samples Submitted to a Veterinary Diagnostic Laboratory in the Midwestern United States.

Authors:  Mehmet Cemal Adiguzel; Kayla Schaefer; Trevor Rodriguez; Jessica Ortiz; Orhan Sahin
Journal:  Antibiotics (Basel)       Date:  2022-04-30

5.  Rapid MRSA detection via tandem mass spectrometry of the intact 80 kDa PBP2a resistance protein.

Authors:  Jason R Neil; Arvind Verma; Scott R Kronewitter; William M McGee; Christopher Mullen; Marjaana Viirtola; Annika Kotovuori; Herdis Friedrich; Johan Finell; Joni Rannisto; John E P Syka; James L Stephenson
Journal:  Sci Rep       Date:  2021-09-15       Impact factor: 4.379

  5 in total

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