| Literature DB >> 27605792 |
Abbas Ali Eskandarian1, Sara Moradi2, Saeed Abedi2.
Abstract
As parasitological or microscopic method is the gold standard and the best method for diagnosis of malaria, so fixed Geimsa-stained blood slides in the form of thick and thin blood smears are the most important data collections of malaria, especially historical slides. The parasites are dead but their DNA is valuable for many molecular biologic researches. A simple and efficient method for catching and extraction malaria parasites' DNA with a desired yield from dried and stained blood on slides is the first and major step. Introduction of an applicable, efficient and inexpensive DNA catching method and assessment of its performance in following molecular applications was the main objective of present study.Entities:
Keywords: Blood slide; DNA extraction; Fixed Giemsa-stained; Malaria; Plasmodium falciparum; Plasmodium vivax
Year: 2014 PMID: 27605792 PMCID: PMC4996200 DOI: 10.1007/s12639-014-0587-3
Source DB: PubMed Journal: J Parasit Dis ISSN: 0971-7196