Literature DB >> 27605792

A simple, efficient and inexpensive method for malaria parasites' DNA catching from fixed Giemsa-stained blood slides.

Abbas Ali Eskandarian1, Sara Moradi2, Saeed Abedi2.   

Abstract

As parasitological or microscopic method is the gold standard and the best method for diagnosis of malaria, so fixed Geimsa-stained blood slides in the form of thick and thin blood smears are the most important data collections of malaria, especially historical slides. The parasites are dead but their DNA is valuable for many molecular biologic researches. A simple and efficient method for catching and extraction malaria parasites' DNA with a desired yield from dried and stained blood on slides is the first and major step. Introduction of an applicable, efficient and inexpensive DNA catching method and assessment of its performance in following molecular applications  was the main objective of present study.

Entities:  

Keywords:  Blood slide; DNA extraction; Fixed Giemsa-stained; Malaria; Plasmodium falciparum; Plasmodium vivax

Year:  2014        PMID: 27605792      PMCID: PMC4996200          DOI: 10.1007/s12639-014-0587-3

Source DB:  PubMed          Journal:  J Parasit Dis        ISSN: 0971-7196


  12 in total

1.  Epidemiologic tools for malaria surveillance in an urban setting of low endemicity along the Colombian Pacific coast.

Authors:  G Carrasquilla; M Banguero; P Sanchez; F Carvajal; R H Barker; G W Gervais; E Algarin; A E Serrano
Journal:  Am J Trop Med Hyg       Date:  2000-01       Impact factor: 2.345

2.  Evaluation of OptiMAL, a dipstick test for the diagnosis of malaria.

Authors:  S M John; A Sudarsanam; U Sitaram; A H Moody
Journal:  Ann Trop Med Parasitol       Date:  1998-07

3.  Detection and identification of the four malaria parasite species infecting humans by PCR amplification.

Authors:  G Snounou
Journal:  Methods Mol Biol       Date:  1996

4.  PCR amplification of DNA from malaria parasites on fixed and stained thick and thin blood films.

Authors:  D Edoh; S Steiger; B Genton; H P Beck
Journal:  Trans R Soc Trop Med Hyg       Date:  1997 May-Jun       Impact factor: 2.184

5.  Novel point mutations in the dihydrofolate reductase gene of Plasmodium vivax: evidence for sequential selection by drug pressure.

Authors:  Mallika Imwong; Sasithon Pukrittayakamee; Laurent Rénia; Franck Letourneur; Jean-Paul Charlieu; Ubolsree Leartsakulpanich; Sornchai Looareesuwan; Nicholas J White; Georges Snounou
Journal:  Antimicrob Agents Chemother       Date:  2003-05       Impact factor: 5.191

6.  High prevalence of Plamodium malariae infections in a Brazilian Amazon endemic area (Apiacás-Mato Grosso State) as detected by polymerase chain reaction.

Authors:  Kézia K G Scopel; Cor J F Fontes; Alvaro C Nunes; M Fátima Horta; Erika M Braga
Journal:  Acta Trop       Date:  2004-03       Impact factor: 3.112

7.  Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections.

Authors:  G Snounou; S Viriyakosol; W Jarra; S Thaithong; K N Brown
Journal:  Mol Biochem Parasitol       Date:  1993-04       Impact factor: 1.759

8.  Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P. vivax infections undetected by conventional microscopy.

Authors:  A E Brown; K C Kain; J Pipithkul; H K Webster
Journal:  Trans R Soc Trop Med Hyg       Date:  1992 Nov-Dec       Impact factor: 2.184

9.  Imported malaria in United Arab Emirates: evaluation of a new DNA extraction technique using nested PCR.

Authors:  Doaa M Sultan; Marwa M Khalil; Ahmed S Abdouh; Wafaa F Doleh; Abdul Aziz M Al Muthanna
Journal:  Korean J Parasitol       Date:  2009-08-28       Impact factor: 1.341

10.  Detection of malaria parasites by nested PCR in south-eastern, Iran: evidence of highly mixed infections in Chahbahar district.

Authors:  Sedigheh Zakeri; Sohaila Talebi Najafabadi; Ahmad Zare; Navid Dinparast Djadid
Journal:  Malar J       Date:  2002-02-08       Impact factor: 2.979

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