Erik Tanis1, Danny J Evers2, Jarich W Spliethoff2, Vishnu V Pully3, Koert Kuhlmann2, Frits van Coevorden2, Benno H W Hendriks3,4, Joyce Sanders5, Warner Prevoo6, Theo J M Ruers2,7. 1. Department of Surgery, The Netherlands Cancer Institute, Amsterdam, The Netherlands. eriktanis@gmail.com. 2. Department of Surgery, The Netherlands Cancer Institute, Amsterdam, The Netherlands. 3. In-Body Systems, Philips Research, Eindhoven, The Netherlands. 4. Delft University of Technology, Delft, The Netherlands. 5. Department of Pathology, The Netherlands Cancer Institute, Amsterdam, The Netherlands. 6. Department of Radiology, The Netherlands Cancer Institute, Amsterdam, The Netherlands. 7. MIRA Institute, University of Twente, Enschede, The Netherlands.
Abstract
BACKGROUND AND OBJECTIVE: Over the last decade, an increasing effort has been put towards the implementation of optical guidance techniques to aid surgeons during cancer surgery. Diffuse reflectance spectroscopy (DRS) and fluorescence spectroscopy (FS) are two of these new techniques. The objective of this study is to investigate whether in vivo optical spectroscopy is able to accurately discriminate colorectal liver metastases (CRLM) from normal liver tissue in vivo. MATERIALS AND METHODS: DRS and FS were incorporated at the tip of a needle and were used for in vivo tissue differentiation during resection of CRLM. Measurements were taken in and around the tumor lesions and measurement sites were marked and correlated to histology (i.e., normal liver tissue or tumor tissue). Patients with and without neoadjuvant systemic chemotherapy were included into the study. RESULTS: Four hundred and eighty-four measurements were taken in and near 19 liver lesions prior to resection. Overall sensitivity and specificity for DRS was 95% and 92%, respectively. Bile was the most discriminative parameter. The addition of FS did not improve the overall accuracy. Sensitivity and specificity was not hampered by neo-adjuvant chemotherapy; sensitivity and specificity after neo-adjuvant chemotherapy were 92% and 100%, respectively. CONCLUSION: We have successfully integrated spectroscopy technology into a disposable 15 Gauge optical needle and we have shown that DRS and FS can accurately discriminate CRLM from normal liver tissue in the in vivo setting regardless of whether the patient was pre-treated with systemic therapy. This technique makes in vivo guidance accessible for common surgical practice. Lasers Surg. Med. 48:820-827, 2016.
BACKGROUND AND OBJECTIVE: Over the last decade, an increasing effort has been put towards the implementation of optical guidance techniques to aid surgeons during cancer surgery. Diffuse reflectance spectroscopy (DRS) and fluorescence spectroscopy (FS) are two of these new techniques. The objective of this study is to investigate whether in vivo optical spectroscopy is able to accurately discriminate colorectal liver metastases (CRLM) from normal liver tissue in vivo. MATERIALS AND METHODS:DRS and FS were incorporated at the tip of a needle and were used for in vivo tissue differentiation during resection of CRLM. Measurements were taken in and around the tumor lesions and measurement sites were marked and correlated to histology (i.e., normal liver tissue or tumor tissue). Patients with and without neoadjuvant systemic chemotherapy were included into the study. RESULTS: Four hundred and eighty-four measurements were taken in and near 19 liver lesions prior to resection. Overall sensitivity and specificity for DRS was 95% and 92%, respectively. Bile was the most discriminative parameter. The addition of FS did not improve the overall accuracy. Sensitivity and specificity was not hampered by neo-adjuvant chemotherapy; sensitivity and specificity after neo-adjuvant chemotherapy were 92% and 100%, respectively. CONCLUSION: We have successfully integrated spectroscopy technology into a disposable 15 Gauge optical needle and we have shown that DRS and FS can accurately discriminate CRLM from normal liver tissue in the in vivo setting regardless of whether the patient was pre-treated with systemic therapy. This technique makes in vivo guidance accessible for common surgical practice. Lasers Surg. Med. 48:820-827, 2016.
Authors: Evgenii A Zherebtsov; Elena V Potapova; Andrian V Mamoshin; Valery V Shupletsov; Ksenia Y Kandurova; Viktor V Dremin; Andrey Y Abramov; Andrey V Dunaev Journal: Biomed Opt Express Date: 2022-01-06 Impact factor: 3.732
Authors: E J M Baltussen; S G Brouwer de Koning; J Sanders; A G J Aalbers; N F M Kok; G L Beets; B H W Hendriks; H J C M Sterenborg; K F D Kuhlmann; T J M Ruers Journal: J Transl Med Date: 2019-10-02 Impact factor: 5.531
Authors: Freija Geldof; Behdad Dashtbozorg; Benno H W Hendriks; Henricus J C M Sterenborg; Theo J M Ruers Journal: Sci Rep Date: 2022-02-01 Impact factor: 4.996