INTRODUCTION: FK506 binding proteins (FKBPs) function as oncogenes or tumor suppressors by interacting with steroid hormone receptors, kinases, or other cellular factors in addition to intracellular ligands FK506 and rapamycin. In this study, we aimed at evaluating the expression and function of FKBPs in renal cell carcinoma (RCC). MATERIALS AND METHODS: Thirty-four RCC specimens were analyzed by whole transcriptome sequencing. Small interfere RNA was employed to knockdown FKBP10 in 786-O and A-704 cells, and cell proliferation, cell cycle progression, invasion and migration were evaluated. RESULTS: FKBP10 was different from other members of FKBPs with most significant upregulation in almost all RCC specimens, compared to normal mucosa. FKBP10 expression was high in additional 38 RCC specimens and RCC cell lines compared to paired non-tumor tissues and normal renal tubule cells. FKBP10 knockdown led to cell cycle arrest at G0/G1 phase, and reduced cell proliferation, invasion, and migration in 786-O and A-704 cells. In addition, heat shock protein 90 was significantly downregulated after FKBP10 knockdown. CONCLUSIONS: FKBP10 is overexpressed and promotes RCC and is a new promising biomarker and therapy target for RCC.
INTRODUCTION:FK506 binding proteins (FKBPs) function as oncogenes or tumor suppressors by interacting with steroid hormone receptors, kinases, or other cellular factors in addition to intracellular ligands FK506 and rapamycin. In this study, we aimed at evaluating the expression and function of FKBPs in renal cell carcinoma (RCC). MATERIALS AND METHODS: Thirty-four RCC specimens were analyzed by whole transcriptome sequencing. Small interfere RNA was employed to knockdown FKBP10 in 786-O and A-704 cells, and cell proliferation, cell cycle progression, invasion and migration were evaluated. RESULTS:FKBP10 was different from other members of FKBPs with most significant upregulation in almost all RCC specimens, compared to normal mucosa. FKBP10 expression was high in additional 38 RCC specimens and RCC cell lines compared to paired non-tumor tissues and normal renal tubule cells. FKBP10 knockdown led to cell cycle arrest at G0/G1 phase, and reduced cell proliferation, invasion, and migration in 786-O and A-704 cells. In addition, heat shock protein 90 was significantly downregulated after FKBP10 knockdown. CONCLUSIONS:FKBP10 is overexpressed and promotes RCC and is a new promising biomarker and therapy target for RCC.
Authors: Marta Sarquis; Debora C Moraes; Luciana Bastos-Rodrigues; Pedro G Azevedo; Adauto V Ramos; Fabiana Versiani Reis; Paula V Dande; Isabela Paim; Eitan Friedman; Luiz De Marco Journal: Endocr Pathol Date: 2020-03 Impact factor: 3.943
Authors: Abdullah Saleh Al-Wajeeh; Salizawati Muhamad Salhimi; Majed Ahmed Al-Mansoub; Imran Abdul Khalid; Thomas Michael Harvey; Aishah Latiff; Mohd Nazri Ismail Journal: PLoS One Date: 2020-01-16 Impact factor: 3.240