Maryam Khalil Arjmandi1, Dariush Moslemi2, Azadeh Sadati Zarrini3, Maryam Ebrahimnezhad Gorji3, Abbas Mosapour1, Asefeh Haghhaghighi1, Sohrab Halalkhor1, Ali Bijani4, Hadi Parsian5. 1. Department of Clinical Biochemistry, Babol University of Medical Sciences, Babol, Iran. 2. Department of Radiation Oncology, Babol University of Medical Sciences, Babol, Iran. 3. Department of Biology, Science and Research Branch, Islamic Azad University, Fars, Iran. 4. Social Determinant of Health Research Center, Babol University of Medical Sciences, Babol, Iran. 5. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Abstract
AIM: In this study the effects of radiation therapy (RT) on serum oxidant/antioxidant status in breast cancer patients and the impact of age, BMI and clinical stage of the disease on the aforementioned variables were investigated. BACKGROUND: RT that is used for cancer treatment is dependent on the production of reactive oxygen species. MATERIALS AND METHODS: Eighty patients with breast cancer participated in this study and received RT at a dose of 50 Gy for 5 weeks. Blood samples were obtained in one day before and after the end of RT. Serum status of malondialdehyde (MDA), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were analyzed by spectrophotometry or ELISA and selenium (Se) level were analyzed by atomic absorption spectrometry. Paired t-test was used for comparing pre and post radiotherapy data. RESULTS: Before and after the radiotherapy, a significant increase in MDA level was observed, while a significant decrease in GPx activity, SOD, TAS and Se levels were found (p < 0.05). The level of the CAT enzyme had no significant changes (p = 0.568). The results showed some changes in the status of TAS, SOD and GPx which are associated with age, BMI and clinical stage of the disease. CONCLUSION: It seems that RT would have the potential to cause variations in the status of antioxidant/oxidant system. Although, some changes in variables were observed by sub-classification of the age, BMI and the disease stage, but it seems that these changes are not necessarily dependent to them.
AIM: In this study the effects of radiation therapy (RT) on serum oxidant/antioxidant status in breast cancerpatients and the impact of age, BMI and clinical stage of the disease on the aforementioned variables were investigated. BACKGROUND: RT that is used for cancer treatment is dependent on the production of reactive oxygen species. MATERIALS AND METHODS: Eighty patients with breast cancer participated in this study and received RT at a dose of 50 Gy for 5 weeks. Blood samples were obtained in one day before and after the end of RT. Serum status of malondialdehyde (MDA), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were analyzed by spectrophotometry or ELISA and selenium (Se) level were analyzed by atomic absorption spectrometry. Paired t-test was used for comparing pre and post radiotherapy data. RESULTS: Before and after the radiotherapy, a significant increase in MDA level was observed, while a significant decrease in GPx activity, SOD, TAS and Se levels were found (p < 0.05). The level of the CAT enzyme had no significant changes (p = 0.568). The results showed some changes in the status of TAS, SOD and GPx which are associated with age, BMI and clinical stage of the disease. CONCLUSION: It seems that RT would have the potential to cause variations in the status of antioxidant/oxidant system. Although, some changes in variables were observed by sub-classification of the age, BMI and the disease stage, but it seems that these changes are not necessarily dependent to them.
Entities:
Keywords:
Breast cancer; Oxidative stress; Radiotherapy; Selenium; Total antioxidant status
Authors: Marika Crohns; Seppo Saarelainen; Hannu Kankaanranta; Eeva Moilanen; Hannu Alho; Pirkko Kellokumpu-Lehtinen Journal: Free Radic Res Date: 2009-07
Authors: Gözde Derindağ; Hayati Murat Akgül; Ahmet Kızıltunç; Halil İbrahim Özkan; Hilal Kızıltunç Özmen; Nilgün Akgül Journal: Turk J Med Sci Date: 2021-04-30 Impact factor: 0.973