Yanmei Zhang1,2,3, Shiwen Wang1, Binjie Hu1, Fuju Zhao1, Ping Xiang4, Danian Ji4, Fei Chen1, Xiaoli Liu5, Feng Yang1, Yong Wu6, Mimi Kong6, Li Nan6, Yingxin Miao1, Wenrong Jiang1, Yi Fang1, Jinghao Zhang1, Zhijun Bao7, Michal A Olszewski8, Hu Zhao1,2,3. 1. Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China. 2. Key Laboratory of Clinical Geriatric Medicine, Shanghai 200040, China. 3. Research Center on Aging & Medicine, Fudan University, Shanghai 200040, China. 4. Department of Endoscopy, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China. 5. Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai 200032, China. 6. Ningbo HEALTH Gene Technologies Co., Ltd, Ningbo 315000, PR China. 7. Department of Gastroenterology, Gerontology Institute of Shanghai affiliated to Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China. 8. Department of Internal Medicine, Division of Pulmonary & Critical Care Medicine, University of Michigan Medical School & Veterans' Affairs Ann Arbor Health System, Ann Arbor, MI 48105, USA.
Abstract
AIM: We evaluated the direct high-throughput multiple genetic detection system (dHMGS) for Helicobacter pylori in gastric biopsies. MATERIALS & METHODS: One hundred and thirty-three specimens were concurrently analyzed by dHMGS, rapid urease test, culture and sequencing. RESULTS: dHMGS was highly sensitive and specific for H. pylori identification compared with culture and rapid urease test. The correlation coefficient of the quantitative standard curve was R2 = 0.983. A significant difference in the relative H. pylori DNA abundance was found in different gastroduodenal diseases. Concordance rates between dHMGS and sequencing for resistance mutations were 97.1, 100.0, 85.3 and 97.1%, respectively. Finally, dHMGS could efficiently distinguish mixed infection in biopsy specimens. CONCLUSION: The dHMGS could efficiently diagnose and quantify H. pylori burden in biopsies, simultaneously screening for virulence, antibiotic resistance and presence of the multistrain infections.
AIM: We evaluated the direct high-throughput multiple genetic detection system (dHMGS) for Helicobacter pylori in gastric biopsies. MATERIALS & METHODS: One hundred and thirty-three specimens were concurrently analyzed by dHMGS, rapid urease test, culture and sequencing. RESULTS:dHMGS was highly sensitive and specific for H. pylori identification compared with culture and rapid urease test. The correlation coefficient of the quantitative standard curve was R2 = 0.983. A significant difference in the relative H. pylori DNA abundance was found in different gastroduodenal diseases. Concordance rates between dHMGS and sequencing for resistance mutations were 97.1, 100.0, 85.3 and 97.1%, respectively. Finally, dHMGS could efficiently distinguish mixed infection in biopsy specimens. CONCLUSION: The dHMGS could efficiently diagnose and quantify H. pylori burden in biopsies, simultaneously screening for virulence, antibiotic resistance and presence of the multistrain infections.