| Literature DB >> 27598229 |
Anoop Kumar Shukla1, Santosh Kumar Upadhyay1, Manisha Mishra1,2, Sharad Saurabh1, Rahul Singh1, Harpal Singh1, Nidhi Thakur1,2, Preeti Rai1, Paras Pandey1,2, Aradhana L Hans1,2, Subhi Srivastava1, Vikram Rajapure1, Sunil Kumar Yadav1,2, Mithlesh Kumar Singh1,2, Jitendra Kumar3, K Chandrashekar1, Praveen C Verma1,2, Ajit Pratap Singh2,4, K N Nair2,4, Smrati Bhadauria5, Muhammad Wahajuddin6, Sarika Singh5, Sharad Sharma5, Ram Sanmukh Upadhyay7, Shirish A Ranade1,2, Rakesh Tuli1, Pradhyumna Kumar Singh1,2.
Abstract
Whitefly (Bemisia tabaci) damages field crops by sucking sap and transmitting viral diseases. None of the insecticidal proteins used in genetically modified (GM) crop plants to date are effective against whitefly. We report the identification of a protein (Tma12) from an edible fern, Tectaria macrodonta (Fee) C. Chr., that is insecticidal to whitefly (median lethal concentration = 1.49 μg/ml in in vitro feeding assays) and interferes with its life cycle at sublethal doses. Transgenic cotton lines that express Tma12 at ∼0.01% of total soluble leaf protein were resistant to whitefly infestation in contained field trials, with no detectable yield penalty. The transgenic cotton lines were also protected from whitefly-borne cotton leaf curl viral disease. Rats fed Tma12 showed no detectable histological or biochemical changes, and this, together with the predicted absence of allergenic domains in Tma12, indicates that Tma12 might be well suited for deployment in GM crops to control whitefly and the viruses it carries.Entities:
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Year: 2016 PMID： 27598229 DOI： 10.1038/nbt.3665
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908