| Literature DB >> 2759518 |
Abstract
A rapid method for the separation of diaphorase (DIA 3) isozymes in the pH range 6-8 is presented. Isoelectric focusing (IEF) of DIA 3 on 0.2 mm thick polyacrylamide gels took 60 min at 5 degrees C. Separated bands are sharp and common phenotypes are readily distinguishable. The method combines high resolution, speed, reproducibility and reagent economy with simplicity.Entities:
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Year: 1989 PMID： 2759518 DOI： 10.1016/0379-0738(89)90205-3
Source DB: PubMed Journal: Forensic Sci Int ISSN： 0379-0738 Impact factor: 2.395