Jinseong Jeon1, Hyuck Jun Mok2, Youngwoo Choi1, Seung Cheol Park2, Hunho Jo3, Jin Her3, Jin-Kwan Han1, Yoon-Keun Kim4, Kwang Pyo Kim2, Changill Ban3. 1. Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Nam-Gu, Pohang, Gyeongbuk, Republic of Korea. 2. Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea. 3. Department of Chemistry, Pohang University of Science and Technology (POSTECH), Nam-Gu, Pohang, Gyeongbuk, Republic of Korea. 4. MD Healthcare, Mapo-gu, Seoul, Republic of Korea.
Abstract
PURPOSE: Extracellular vesicle (EV) has been reported to conduct critical pathophysiological functions as an emerging mode of communication in bacteria. Recently, Propionibacterium acnes, an anaerobic Gram-positive human commensal found in the skin and gastrointestinal tract, has drawn increasing attention as an underestimated pathogen in a variety of diseases. EXPERIMENTAL DESIGN: For the comprehensive understanding of P. acnes, here we report the isolation of P. acnes EVs for the first time and identification of 252 vesicular proteins with high confidence using triplicate LC-MS/MS analyses. RESULT: Comprehensive proteomic profiling reveals that P. acnes EVs harbor various proteins involved in biochemical processes, antibiotic resistance, bacterial competition, cell adherence, virulence, and immunogenicity. CONCLUSION AND CLINICAL RELEVANCE: We believe that this report will provide valuable information for investigating the biological role of P. acnes EVs and effective targets for developing clinical applications against P. acnes.
PURPOSE: Extracellular vesicle (EV) has been reported to conduct critical pathophysiological functions as an emerging mode of communication in bacteria. Recently, Propionibacterium acnes, an anaerobic Gram-positive human commensal found in the skin and gastrointestinal tract, has drawn increasing attention as an underestimated pathogen in a variety of diseases. EXPERIMENTAL DESIGN: For the comprehensive understanding of P. acnes, here we report the isolation of P. acnes EVs for the first time and identification of 252 vesicular proteins with high confidence using triplicate LC-MS/MS analyses. RESULT: Comprehensive proteomic profiling reveals that P. acnes EVs harbor various proteins involved in biochemical processes, antibiotic resistance, bacterial competition, cell adherence, virulence, and immunogenicity. CONCLUSION AND CLINICAL RELEVANCE: We believe that this report will provide valuable information for investigating the biological role of P. acnes EVs and effective targets for developing clinical applications against P. acnes.
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