| Literature DB >> 27594413 |
Anuradha Kalani1, Pankaj Chaturvedi2, Pradip K Kamat2, Claudio Maldonado3, Philip Bauer4, Irving G Joshua2, Suresh C Tyagi2, Neetu Tyagi2.
Abstract
We tested whether the combined nano-formulation, prepared with curcumin (anti-inflammatory and neuroprotective molecule) and embryonic stem cell exosomes (MESC-exocur), restored neurovascular loss following an ischemia reperfusion (IR) injury in mice. IR-injury was created in 8-10 weeks old mice and divided into two groups. Out of two IR-injured groups, one group received intranasal administration of MESC-exocur for 7days. Similarly, two sham groups were made and one group received MESC-exocur treatment. The study determined that MESC-exocur treatment reduced neurological score, infarct volume and edema following IR-injury. As compared to untreated IR group, MESC-exocur treated-IR group showed reduced inflammation and N-methyl-d-aspartate receptor expression. Treatment of MESC-exocur also reduced astrocytic GFAP expression and alleviated the expression of NeuN positive neurons in IR-injured mice. In addition, MESC-exocur treatment restored vascular endothelial tight (claudin-5 and occludin) and adherent (VE-cadherin) junction proteins in IR-injured mice as compared to untreated IR-injured mice. These results suggest that combining the potentials of embryonic stem cell exosomes and curcumin can help neurovascular restoration following ischemia-reperfusion injury in mice.Entities:
Keywords: Curcumin; Exosomes; Neurons; Stroke
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Year: 2016 PMID： 27594413 PMCID： PMC5067233 DOI： 10.1016/j.biocel.2016.09.002
Source DB: PubMed Journal: Int J Biochem Cell Biol ISSN： 1357-2725 Impact factor: 5.085