Hakan Turkon 1 , Burak Toprak 2 , Hulya Yalcin 3 , Ayfer Colak 3 , Nurinnisa Ozturk 4 Show Affiliations »
Abstract
OBJECTIVE: To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability. METHODS: We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: see text] for 0, 2, 4, 8, 24, and 72 hours. ACTH concentrations in 4 storage conditions were compared with repeated-measures analysis of variance (ANOVA) at each time point. RESULTS: ACTH concentrations substantially decreased after a 72-hour time period. At the 72nd hour, standard EDTA tubes and EDTA + aprotinin tubes stored at 4[Formula: see text] showed the optimal stability. EDTA + aprotinin tubes stored at room temperature performed more strongly than standard EDTA tubes stored at room temperature. CONCLUSION: Our results show that refrigerated storage is more effective than protease inhibitor addition to maintain ACTH stability. We recommend using aprotinin tubes, especially for specimens standing at room temperature. © American Society for Clinical Pathology, 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
OBJECTIVE: To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability. METHODS: We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid ) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: see text] for 0, 2, 4, 8, 24, and 72 hours. ACTH concentrations in 4 storage conditions were compared with repeated-measures analysis of variance (ANOVA) at each time point. RESULTS: ACTH concentrations substantially decreased after a 72-hour time period. At the 72nd hour, standard EDTA tubes and EDTA + aprotinin tubes stored at 4[Formula: see text] showed the optimal stability. EDTA + aprotinin tubes stored at room temperature performed more strongly than standard EDTA tubes stored at room temperature. CONCLUSION: Our results show that refrigerated storage is more effective than protease inhibitor addition to maintain ACTH stability. We recommend using aprotinin tubes, especially for specimens standing at room temperature. © American Society for Clinical Pathology, 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Entities: Chemical
Keywords:
ACTH; aprotinin; preanalytical phase; proteases; stability; temperature
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Year: 2016
PMID： 27593170 DOI： 10.1093/labmed/lmw027
Source DB: PubMed Journal: Lab Med ISSN： 0007-5027