Martin-Immanuel Bittner1,2,3, Nicole Wiedenmann1,3, Sabine Bucher1,3, Michael Hentschel1,3,4, Michael Mix3,5, Gerta Rücker6, Wolfgang A Weber3,5,7, Philipp T Meyer3,5, Martin Werner3,8, Anca-Ligia Grosu1,3, Gian Kayser3,8. 1. a Department of Radiation Oncology , Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg , Germany. 2. b CRUK/MRC Oxford Institute for Radiation Oncology , University of Oxford , UK. 3. c German Cancer Consortium (DKTK) , Partner Site Freiburg and German Cancer Research Center (DKFZ) , Heidelberg , Germany. 4. d Department of Nuclear Medicine , Inselspital Bern , Bern , Switzerland. 5. e Department of Nuclear Medicine , Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg , Germany. 6. f Institute for Medical Biometry and Statistics , Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg , Germany. 7. g Molecular Imaging and Therapy Service , Memorial Sloan-Kettering Cancer Center , New York , USA. 8. h Institute of Surgical Pathology, Department of Pathology , Medical Center?University of Freiburg, Faculty of Medicine, University of Freiburg , Germany.
Abstract
BACKGROUND: Tumor hypoxia is associated with poor prognosis and outcome and can be visualized using 18F-MISO-positron emission tomography (PET) imaging. The goal of this study was to evaluate the correlation between biological markers and biological imaging in a group of patients in whom a correlation between biological imaging and outcome has previously been demonstrated. MATERIAL AND METHODS: In a prospective pilot project, 16 patients with locally advanced cancer of the head and neck underwent 18F-MISO-PET scans before and during primary radiochemotherapy in addition to 18F-FDG-PET and computed tomography (CT). Tumor biopsies were stained for three tissue-based markers (Ku80, CAIX, CD44); in addition, human papillomavirus (HPV) status was assessed. H-scores of marker expression were generated and the results were correlated with the biological imaging and clinical outcome. RESULTS: No statistically significant correlation was established between the H-scores for Ku80, CD44 and CAIX or between any of the H-scores and the imaging variables (tumor volume on 18F-FDG-PET in ml, hypoxic subvolume as assessed by 18F-MISO-PET in ml, and SUVmax tumor/SUVmean muscle during the 18F-MISO-PET). A statistically significant negative correlation was found between CD44 H-score and HPV status (p = .004). Cox regression analysis for overall survival and recurrence-free survival showed one significant result for CAIX being associated with improved overall survival [hazard ratio 0.96 (0.93-1.00), p = .047]. CONCLUSION: Expression of Ku80, CAIX and CD44 as assessed by immunohistochemistry of tumor biopsies were not correlated to one another or the biological imaging data. However, there was a significant influence of CAIX on overall survival and between CD44 and HPV.
BACKGROUND:Tumor hypoxia is associated with poor prognosis and outcome and can be visualized using 18F-MISO-positron emission tomography (PET) imaging. The goal of this study was to evaluate the correlation between biological markers and biological imaging in a group of patients in whom a correlation between biological imaging and outcome has previously been demonstrated. MATERIAL AND METHODS: In a prospective pilot project, 16 patients with locally advanced cancer of the head and neck underwent 18F-MISO-PET scans before and during primary radiochemotherapy in addition to 18F-FDG-PET and computed tomography (CT). Tumor biopsies were stained for three tissue-based markers (Ku80, CAIX, CD44); in addition, human papillomavirus (HPV) status was assessed. H-scores of marker expression were generated and the results were correlated with the biological imaging and clinical outcome. RESULTS: No statistically significant correlation was established between the H-scores for Ku80, CD44 and CAIX or between any of the H-scores and the imaging variables (tumor volume on 18F-FDG-PET in ml, hypoxic subvolume as assessed by 18F-MISO-PET in ml, and SUVmax tumor/SUVmean muscle during the 18F-MISO-PET). A statistically significant negative correlation was found between CD44 H-score and HPV status (p = .004). Cox regression analysis for overall survival and recurrence-free survival showed one significant result for CAIX being associated with improved overall survival [hazard ratio 0.96 (0.93-1.00), p = .047]. CONCLUSION: Expression of Ku80, CAIX and CD44 as assessed by immunohistochemistry of tumor biopsies were not correlated to one another or the biological imaging data. However, there was a significant influence of CAIX on overall survival and between CD44 and HPV.
Authors: Nils H Nicolay; Nicole Wiedenmann; Michael Mix; Wolfgang A Weber; Martin Werner; Anca L Grosu; Gian Kayser Journal: Eur J Nucl Med Mol Imaging Date: 2019-12-07 Impact factor: 9.236
Authors: A Sörensen; M Carles; H Bunea; L Majerus; C Stoykow; N H Nicolay; N E Wiedenmann; P Vaupel; P T Meyer; A L Grosu; M Mix Journal: Eur J Nucl Med Mol Imaging Date: 2019-11-26 Impact factor: 9.236
Authors: Nicole Wiedenmann; Hatice Bunea; Hans C Rischke; Andrei Bunea; Liette Majerus; Lars Bielak; Alexey Protopopov; Ute Ludwig; Martin Büchert; Christian Stoykow; Nils H Nicolay; Wolfgang A Weber; Michael Mix; Philipp T Meyer; Jürgen Hennig; Michael Bock; Anca L Grosu Journal: Radiat Oncol Date: 2018-08-29 Impact factor: 3.481
Authors: Lars Bielak; Nicole Wiedenmann; Nils Henrik Nicolay; Thomas Lottner; Johannes Fischer; Hatice Bunea; Anca-Ligia Grosu; Michael Bock Journal: Tomography Date: 2019-09