Zhiqiang Zhang 1 , Cong Li 2 , Qijia Tan 2 , Caijun Xie 2 , Yanying Yang 2 , Wengang Zhan 2 , Fu Han 2 , Hari Shanker Sharma 1 , Aruna Sharma 3 Show Affiliations »
Abstract
PURPOSE: To investigate the effect of curcumin on tumor growth and angiogenesis of human gliomas and identify the underlying molecular mechanisms. METHODS: A mouse xenograft glioma model was established by subcutaneously inoculating tumor cell aggregates derived from the U87 cell line. Mice were treated with 0.01ml/g body weight of curcumin or saline. Tumor volume was measured. Microvessel density was assessed by CD34 immunostaining, and angiogenesis by immunohistochemical staining of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2) and thrombospondin 1 (TSP-1). RESULTS: At 28 days after treatment, tumor weights in the curcumin-treated group were much smaller than in the control group (0.23±0.11g vs 0.44±0.15g，p＜0.05), resulting in a 45.8% inhibition of tumor growth. Curcumin also markedly inhibited microvessel density. Expression of VEGF and Ang-2 was inhibited by curcumin, whereas TSP-1 expression was up-regulated. CONCLUSION: This study shows that curcumin inhibits tumor growth by inhibiting VEGF/Ang-2/TSP-1- mediated angiogenesis in a xenograft glioma mouse model. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.
PURPOSE: To investigate the effect of curcumin on tumor growth and angiogenesis of human gliomas and identify the underlying molecular mechanisms. METHODS: A mouse xenograft glioma model was established by subcutaneously inoculating tumor cell aggregates derived from the U87 cell line. Mice were treated with 0.01ml/g body weight of curcumin or saline . Tumor volume was measured. Microvessel density was assessed by CD34 immunostaining, and angiogenesis by immunohistochemical staining of vascular endothelial growth factor (VEGF ), angiopoietin-2 (Ang-2 ) and thrombospondin 1 (TSP-1 ). RESULTS: At 28 days after treatment, tumor weights in the curcumin -treated group were much smaller than in the control group (0.23±0.11g vs 0.44±0.15g，p＜0.05), resulting in a 45.8% inhibition of tumor growth. Curcumin also markedly inhibited microvessel density. Expression of VEGF and Ang-2 was inhibited by curcumin , whereas TSP-1 expression was up-regulated. CONCLUSION: This study shows that curcumin inhibits tumor growth by inhibiting VEGF /Ang-2 /TSP-1 - mediated angiogenesis in a xenograft glioma mouse model. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.
Entities: Chemical
Disease
Gene
Species
Keywords:
Curcumin; angiogenesis; glioma; vascular endothelial growth factor; vascular endothelial growth factor.Curcumin
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Year: 2017
PMID： 27592626 DOI： 10.2174/1871527315666160902144513
Source DB: PubMed Journal: CNS Neurol Disord Drug Targets ISSN： 1871-5273 Impact factor: 4.388