Mar Olga Pérez-Moreno1, María José Centelles-Serrano2, Julio Nogales-López3, Marie France Domenech-Spanedda4, Carmen Lozano5, Carmen Torres6. 1. Laboratori Clínic ICS-Terres de l'Ebre (Área de microbiología), Hospital de Tortosa Verge de la Cinta, Tortosa (Tarragona), Spain; Institut d'Investigació Sanitària Pere Virgili (IISPV), Spain. Electronic address: moperez.ebre.ics@gencat.cat. 2. Laboratori Clínic ICS-Terres de l'Ebre (Área de microbiología), Hospital de Tortosa Verge de la Cinta, Tortosa (Tarragona), Spain; Institut d'Investigació Sanitària Pere Virgili (IISPV), Spain. 3. Servicio de Cirugía Ortopédica y Traumatología, Hospital de Tortosa Verge de la Cinta, Tortosa (Tarragona), Spain. 4. Servicio de Medicina Preventiva, Hospital de Tortosa Verge de la Cinta, Tortosa (Tarragona), Spain. 5. Centro de Investigación Biomédica de la Rioja, Área de Microbiología Molecular, Logroño, Spain. 6. Facultad de Ciencia y Tecnología, Área de Bioquímica y Biología Molecular, Universidad de la Rioja, Logroño, Spain.
Abstract
OBJECTIVE: To characterize a methicillin-resistant Staphylococcus aureus (MRSA) isolate responsible for an aggressive infection (peridural and psoas abscess secondary to haematogenous septic arthritis) in a poultry farmer. METHODS: Molecular characterization was performed, including spa- and multilocus sequence typing of the isolate, assessment of its resistance phenotype and detection of tetracycline resistance and of virulence and immune evasion cluster (IEC) genes were performed. RESULTS: The MRSA isolate was tetracycline- and fluorquinolone-resistant, and was ascribed to CC398, spa-t1451. The isolate harboured tet(M) (distinctive of livestock-associated (LA) MRSA-CC398 clade) and IEC-type B system (characteristic of the methicillin-susceptible human lineage, but typically absent in LA-MRSA-CC398 strains), and lacked toxin-coding genes lukF/lukS-PV, tsst-1, eta and etb. CONCLUSION: IEC re-acquisition by LA-MRSA-CC398-LA strains is an unusual finding, but could constitute an emerging public health problem. It would represent an evolutionary step towards LA-MRSA-CC398's adaptation to human hosts, and might enhance its invasiveness and ability to be transmitted to humans.
OBJECTIVE: To characterize a methicillin-resistant Staphylococcus aureus (MRSA) isolate responsible for an aggressive infection (peridural and psoas abscess secondary to haematogenous septic arthritis) in a poultry farmer. METHODS: Molecular characterization was performed, including spa- and multilocus sequence typing of the isolate, assessment of its resistance phenotype and detection of tetracycline resistance and of virulence and immune evasion cluster (IEC) genes were performed. RESULTS: The MRSA isolate was tetracycline- and fluorquinolone-resistant, and was ascribed to CC398, spa-t1451. The isolate harboured tet(M) (distinctive of livestock-associated (LA) MRSA-CC398 clade) and IEC-type B system (characteristic of the methicillin-susceptible human lineage, but typically absent in LA-MRSA-CC398 strains), and lacked toxin-coding genes lukF/lukS-PV, tsst-1, eta and etb. CONCLUSION: IEC re-acquisition by LA-MRSA-CC398-LA strains is an unusual finding, but could constitute an emerging public health problem. It would represent an evolutionary step towards LA-MRSA-CC398's adaptation to human hosts, and might enhance its invasiveness and ability to be transmitted to humans.
Keywords:
Absceso epidural y de psoas; Artritis séptica hematógena; Haematogenous septic arthritis; Immune evasion cluster; Livestock-associated MRSA-CC398; Peridural and psoas abscess; SARM-CC398 asociado al ganado; Sistema de evasión inmune
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