Genome Sequence of Aeromonas hydrophila Strain AH-3 (Serotype O34).

Aeromonas hydrophila is an emerging pathogen of poikilothermic animals, from fish to mammals, including humans. Here, we report the whole-genome sequence of the A. hydrophila AH-3 strain, isolated from a fish farm goldfish septicemia outbreak in Spain, with a characterized polar and lateral flagellum glycosylation pattern.

GENOME ANNOUNCEMENT

Much evidence in recent years has shown that protein glycosylation, once thought to be a eukaryote-only posttranslational modification, is not only present in bacteria but also plays a key role in bacterial infectivity and virulence (1, 2). Protein glycosylation in bacteria involves mainly surface array proteins and flagellin, although the mechanisms governing this phenomenon are not completely elucidated and appear to be species specific (3).

Aeromonas hydrophila AH-3, which was recently classified as Aeromonas piscicola AH-3 (4), is linked to gastroenteric infections and has a different and characterized glycosylation pattern in both the polar and lateral flagella (5). Similar to other well-known bacterial virulence factors, flagellar glycosylation on this strain affects both bacterial colonization and the inflammatory response in human cultured cells (3). The analysis of the genome from this strain will allow in-depth understanding of the genes involved in the glycosylation process in bacteria and its importance in virulence and pathogenicity.

The genome of A. hydrophila strain AH-3 was fully sequenced using Illumina MiSeq II, generating a total of 5,456,534 paired reads with 84× coverage. Read quality analysis and trimming were done with Prinseq 0.20.4 (6). De novo assembly with SPAdes 3.6.0 (7) resulted in 82 scaffolds larger than 500 kb.

Genome annotation was performed both via the NCBI Prokaryotic Genome Annotation Pipeline (PGAAP) and Rapid Annotations using Subsystems Technology (RAST). The complete genome of A. hydrophila AH-3 is 4,886,878 bp, with 59.8% G+C content, and codes for 4,399 predicted genes, five rRNAs, and 79 tRNA sequences.

Accession number(s).

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number LYXO00000000 (BioProject PRJNA323710). The version described in this paper is the first version, LYXO01000000.