Jung-Hee Jang1, Deuk-Joo Lee1, Chang-Hwan Bae1, Ki-Tae Ha1, Sunoh Kwon2,3, Hi-Joon Park2, Dae-Hyun Hahm2, Hyejung Lee2, Seungtae Kim4. 1. School of Korean Medicine, Pusan National University, Yangsan, 50612, Republic of Korea. 2. Acupuncture and Meridian Science Research Center, Kyung Hee University, Seoul, 02447, Republic of Korea. 3. Department of Psychiatry and Behavioral Sciences, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA. 4. School of Korean Medicine, Pusan National University, Yangsan, 50612, Republic of Korea. kimst@pusan.ac.kr.
Abstract
OBJECTIVES: To clarify the effects of acupuncture stimulation at Zusanli (ST 36) on the hormonal changes. METHODS: Eight-week-old male C57BL/6 mice received acupuncture stimulation at acupoint ST 36 or Quchi (LI 11) once a day for 3 or 5 days in the acupuncture-stimulated groups, but not received in the normal group (n=6 in each group). On day 3 or 5, animals were given 0.1 mL of charcoal orally with a bulbed steel needle, 30 min after the last acupuncture stimulation. Ten minutes later, mice were anesthetized, and the intestinal transit and the concentrations of vasoactive intestinal peptide (VIP), motilin, ghrelin and gastrin in the serum were measured. RESULTS: Compared to no acupuncture stimulation, acupuncture stimulation at ST 36 for 5 days increased the intestinal transit and down-regulated the concentration of VIP and up-regulated the concentrations of motilin, ghrelin and gastrin (P<0.05 or 0.01), whereas acupuncture stimulation at LI 11 did not change them signifificantly (P>0.05). CONCLUSION: Acupuncture stimulation at ST 36 for 5 days enhances the small intestinal motility and regulates the secretion of hormones related to small intestinal motility.
OBJECTIVES: To clarify the effects of acupuncture stimulation at Zusanli (ST 36) on the hormonal changes. METHODS: Eight-week-old male C57BL/6 mice received acupuncture stimulation at acupoint ST 36 or Quchi (LI 11) once a day for 3 or 5 days in the acupuncture-stimulated groups, but not received in the normal group (n=6 in each group). On day 3 or 5, animals were given 0.1 mL of charcoal orally with a bulbed steel needle, 30 min after the last acupuncture stimulation. Ten minutes later, mice were anesthetized, and the intestinal transit and the concentrations of vasoactive intestinal peptide (VIP), motilin, ghrelin and gastrin in the serum were measured. RESULTS: Compared to no acupuncture stimulation, acupuncture stimulation at ST 36 for 5 days increased the intestinal transit and down-regulated the concentration of VIP and up-regulated the concentrations of motilin, ghrelin and gastrin (P<0.05 or 0.01), whereas acupuncture stimulation at LI 11 did not change them signifificantly (P>0.05). CONCLUSION: Acupuncture stimulation at ST 36 for 5 days enhances the small intestinal motility and regulates the secretion of hormones related to small intestinal motility.