| Literature DB >> 2758580 |
R A Magnotti1, G W Stephens, R K Rogers, A J Pesce.
Abstract
We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of creatinine in urine and plasma samples with a commercially available enzymatic kit modified for analysis by use of an inexpensive microplate reader produced values comparable in precision and accuracy to those obtained by an automated kinetic Jaffé method.Entities:
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Year: 1989 PMID: 2758580
Source DB: PubMed Journal: Clin Chem ISSN: 0009-9147 Impact factor: 8.327