Saranya Chandrudu1, Stacey Bartlett1, Zeinab G Khalil2, Zhongfan Jia3, Waleed M Hussein1, Robert J Capon2, Michael R Batzloff4, Michael F Good4, Michael J Monteiro3, Mariusz Skwarczynski1, Istvan Toth1,2,5. 1. School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia. 2. Institute for Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia. 3. Australian Institute for Bioengineering & Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia. 4. Institute for Glycomics, Griffith University, Gold Coast, QLD 4222, Australia. 5. School of Pharmacy, The University of Queensland, Woolloongabba, QLD 4102, Australia.
Abstract
AIM: Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system. RESULTS: Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper (PADRE) epitope, were conjugated to a variety of linear and branched polyacrylates. All produced conjugates formed submicron-sized particles and induced a high level of IgG titres in mice after subcutaneous immunization. These polymer-peptide conjugates demonstrated high opsonization capacity against group A streptococcus clinical isolates. CONCLUSION: We have successfully demonstrated that submicron-sized polymer-peptide conjugates were capable of inducing strong humoral immune responses after single immunization.
AIM: Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system. RESULTS: Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper (PADRE) epitope, were conjugated to a variety of linear and branched polyacrylates. All produced conjugates formed submicron-sized particles and induced a high level of IgG titres in mice after subcutaneous immunization. These polymer-peptide conjugates demonstrated high opsonization capacity against group A streptococcus clinical isolates. CONCLUSION: We have successfully demonstrated that submicron-sized polymer-peptide conjugates were capable of inducing strong humoral immune responses after single immunization.
Entities:
Keywords:
clinical isolates; group A streptococcus; nanoparticles; opsonization; peptide vaccine; polyacrylates; polymer–peptide conjugate; single immunization; vaccine delivery
Authors: Mariusz Skwarczynski; Guangzu Zhao; Jennifer C Boer; Victoria Ozberk; Armira Azuar; Jazmina Gonzalez Cruz; Ashwini Kumar Giddam; Zeinab G Khalil; Manisha Pandey; Mohini A Shibu; Waleed M Hussein; Reshma J Nevagi; Michael R Batzloff; James W Wells; Robert J Capon; Magdalena Plebanski; Michael F Good; Istvan Toth Journal: Sci Adv Date: 2020-01-29 Impact factor: 14.136