Literature DB >> 27581010

Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible.

Hidetoshi Mori1, Robert D Cardiff2.   

Abstract

Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the "pros and cons" in each method, along with alternative procedures and the suggested imaging equipment.

Keywords:  Autofluorescence; Formalin-fixed paraffin-embedded tissues; Immunofluorescence; Immunohistochemistry; Zinc fixation

Mesh:

Year:  2016        PMID: 27581010     DOI: 10.1007/978-1-4939-3801-8_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  15 in total

1.  In Situ Observation of Abscisic Acid Distribution in Major Crop Species by Immunofluorescence Labeling.

Authors:  Mo-Xian Chen; Chong-Chong Lu; Jianhua Zhang; Ying-Gao Liu
Journal:  Methods Mol Biol       Date:  2022

2.  In Silico Multi-Compartment Detection Based on Multiplex Immunohistochemical Staining in Renal Pathology.

Authors:  Kuang-Yu Jen; Leema Krishna Murali; Brendon Lutnick; Brandon Ginley; Darshana Govind; Hidetoshi Mori; Guofeng Gao; Pinaki Sarder
Journal:  Proc SPIE Int Soc Opt Eng       Date:  2021-02-15

3.  ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer.

Authors:  Feng He; Hang Xiao; Yixin Cai; Ni Zhang
Journal:  Cell Commun Signal       Date:  2021-05-12       Impact factor: 5.712

4.  PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer.

Authors:  Hong-Kun Wu; Chang Liu; Xin-Xing Li; Wei Ji; Chen-De Xin; Zhi-Qian Hu; Lin Zhou
Journal:  Aging (Albany NY)       Date:  2020-07-07       Impact factor: 5.682

Review 5.  Neurodegenerative Proteinopathies in the Proteoform Spectrum-Tools and Challenges.

Authors:  Aneeqa Noor; Saima Zafar; Inga Zerr
Journal:  Int J Mol Sci       Date:  2021-01-22       Impact factor: 5.923

6.  Emodin Alleviates Severe Acute Pancreatitis-Associated Acute Lung Injury by Inhibiting the Cold-Inducible RNA-Binding Protein (CIRP)-Mediated Activation of the NLRP3/IL-1β/CXCL1 Signaling.

Authors:  Qiushi Xu; Mengfei Wang; Haoya Guo; Huanhuan Liu; Guixin Zhang; Caiming Xu; Hailong Chen
Journal:  Front Pharmacol       Date:  2021-04-23       Impact factor: 5.810

7.  Identification of the Key Immune-Related Genes in Chronic Obstructive Pulmonary Disease Based on Immune Infiltration Analysis.

Authors:  Hongqiong Meng; Qionghua Long; Ruiping Wang; Xian Zhou; Huipeng Su; Tingting Wang; Ya Li
Journal:  Int J Chron Obstruct Pulmon Dis       Date:  2022-01-04

8.  Synaptic Protein Phosphorylation Networks Are Associated With Electroacupuncture-Induced Circadian Control in the Suprachiasmatic Nucleus.

Authors:  Xiaoxiao Lu; Minjie Zhou; Nannan Liu; Chengshun Zhang; Zhengyu Zhao; Dingjun Cai
Journal:  Front Genet       Date:  2021-12-16       Impact factor: 4.599

9.  In vivo visualization and characterization of inflamed intestinal wall: the exploration of targeted microbubbles in assessing NF-κB expression.

Authors:  Chenyang Zhao; Li Ma; Yanwen Luo; Wenbo Li; Mengsu Xiao; Qingli Zhu; Yuxin Jiang
Journal:  J Cell Mol Med       Date:  2021-08-19       Impact factor: 5.310

10.  HER2 Isoforms Uniquely Program Intratumor Heterogeneity and Predetermine Breast Cancer Trajectories During the Occult Tumorigenic Phase.

Authors:  Joshua D Ginzel; Chaitanya R Acharya; Veronica Lubkov; Robert D Cardiff; Alexander D Borowsky; H Kim Lyerly; Joshua C Snyder; Hidetoshi Mori; Peter G Boone; Lauren K Rochelle; Wendy L Roberts; Jeffrey I Everitt; Zachary C Hartman; Erika J Crosby; Lawrence S Barak; Marc G Caron; Jane Q Chen; Neil E Hubbard
Journal:  Mol Cancer Res       Date:  2021-06-15       Impact factor: 5.852

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