Literature DB >> 27580706

Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation.

Dawn L Brasaemle1, Nathan E Wolins2.   

Abstract

Lipid droplets are organelles found in most mammalian cells, as well as in various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol in which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation. © 2016 by John Wiley & Sons, Inc.
Copyright © 2016 John Wiley & Sons, Inc.

Entities:  

Keywords:  cell cultures; density-gradient centrifugation; isolation of lipid droplets; mammalian; preparation of lipid droplets by centrifugation

Mesh：

Substances：

Year:  2016        PMID： 27580706      PMCID： PMC5064949          DOI： 10.1002/cpcb.10

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  19 in total

Review 1.  Enzymes of triacylglycerol synthesis and their regulation.

Authors:  Rosalind A Coleman; Douglas P Lee
Journal:  Prog Lipid Res       Date:  2004-03       Impact factor: 16.195

Review 2.  The proteomics of lipid droplets: structure, dynamics, and functions of the organelle conserved from bacteria to humans.

Authors:  Li Yang; Yunfeng Ding; Yong Chen; Shuyan Zhang; Chaoxing Huo; Yang Wang; Jinhai Yu; Peng Zhang; Huimin Na; Huina Zhang; Yanbin Ma; Pingsheng Liu
Journal:  J Lipid Res       Date:  2012-04-25       Impact factor: 5.922

3.  Fatty acid trafficking in starved cells: regulation by lipid droplet lipolysis, autophagy, and mitochondrial fusion dynamics.

Authors:  Angelika S Rambold; Sarah Cohen; Jennifer Lippincott-Schwartz
Journal:  Dev Cell       Date:  2015-03-05       Impact factor: 12.270

4.  Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein.

Authors:  D L Brasaemle; T Barber; N E Wolins; G Serrero; E J Blanchette-Mackie; C Londos
Journal:  J Lipid Res       Date:  1997-11       Impact factor: 5.922

Review 5.  Packaging of fat: an evolving model of lipid droplet assembly and expansion.

Authors:  Dawn L Brasaemle; Nathan E Wolins
Journal:  J Biol Chem       Date:  2011-11-16       Impact factor: 5.157

6.  A simple and rapid method to assay triacylglycerol in cells and tissues.

Authors:  Danielle M Schwartz; Nathan E Wolins
Journal:  J Lipid Res       Date:  2007-08-23       Impact factor: 5.922

Review 7.  Demonstrated and inferred metabolism associated with cytosolic lipid droplets.

Authors:  Joel M Goodman
Journal:  J Lipid Res       Date:  2009-08-20       Impact factor: 5.922

8.  Factors underlying the variability of lipid droplet fluorescence in MA-10 Leydig tumor cells.

Authors:  P M Gocze; D A Freeman
Journal:  Cytometry       Date:  1994-10-01

Review 9.  Lipid droplet biogenesis.

Authors:  Florian Wilfling; Joel T Haas; Tobias C Walther; Robert V Farese
Journal:  Curr Opin Cell Biol       Date:  2014-04-14       Impact factor: 8.382

10.  Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

Authors:  Y Fujiki; A L Hubbard; S Fowler; P B Lazarow
Journal:  J Cell Biol       Date:  1982-04       Impact factor: 10.539
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  18 in total

1.  Proteomic analysis of monolayer-integrated proteins on lipid droplets identifies amphipathic interfacial α-helical membrane anchors.

Authors:  Camille I Pataki; João Rodrigues; Lichao Zhang; Junyang Qian; Bradley Efron; Trevor Hastie; Joshua E Elias; Michael Levitt; Ron R Kopito
Journal:  Proc Natl Acad Sci U S A       Date:  2018-08-13       Impact factor: 11.205

2.  Rickettsia conorii survival in THP-1 macrophages involves host lipid droplet alterations and active rickettsial protein production.

Authors:  Paige E Allen; Robert C Noland; Juan J Martinez
Journal:  Cell Microbiol       Date:  2021-09-13       Impact factor: 3.715

3.  β-Adrenergic induction of lipolysis in hepatocytes is inhibited by ethanol exposure.

Authors:  Micah B Schott; Karuna Rasineni; Shaun G Weller; Ryan J Schulze; Arthur C Sletten; Carol A Casey; Mark A McNiven
Journal:  J Biol Chem       Date:  2017-05-17       Impact factor: 5.157

4.  Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit.

Authors:  Jascha Brettschneider; Jason M Correnti; Chelsea Lin; Bianca Williams; Amanke Oranu; Amy Kuriakose; Dru McIver-Jenkins; Abigail Haba; Isabelle Kaneza; Sookyoung Jeon; Eleonora Scorletti; Rotonya M Carr
Journal:  J Vis Exp       Date:  2019-04-19       Impact factor: 1.355

Review 5.  Toward Exosome-Based Neuronal Diagnostic Devices.

Authors:  Yong Kyoung Yoo; Junwoo Lee; Hyungsuk Kim; Kyo Seon Hwang; Dae Sung Yoon; Jeong Hoon Lee
Journal:  Micromachines (Basel)       Date:  2018-11-29       Impact factor: 2.891

6.  Lipid droplet size directs lipolysis and lipophagy catabolism in hepatocytes.

Authors:  Micah B Schott; Shaun G Weller; Ryan J Schulze; Eugene W Krueger; Kristina Drizyte-Miller; Carol A Casey; Mark A McNiven
Journal:  J Cell Biol       Date:  2019-08-07       Impact factor: 10.539

7.  Proteomic Characterization of Cytoplasmic Lipid Droplets in Human Metastatic Breast Cancer Cells.

Authors:  Alyssa S Zembroski; Chaylen Andolino; Kimberly K Buhman; Dorothy Teegarden
Journal:  Front Oncol       Date:  2021-06-01       Impact factor: 6.244

8.  The phospholipase PNPLA7 functions as a lysophosphatidylcholine hydrolase and interacts with lipid droplets through its catalytic domain.

Authors:  Christoph Heier; Benedikt Kien; Feifei Huang; Thomas O Eichmann; Hao Xie; Rudolf Zechner; Ping-An Chang
Journal:  J Biol Chem       Date:  2017-09-07       Impact factor: 5.157

9.  Targeting lipid droplet lysophosphatidylcholine for cisplatin chemotherapy.

Authors:  Lumin Chen; Wen-Lung Ma; Wei-Chung Cheng; Juan-Cheng Yang; Hsiao-Ching Wang; Yu-Ting Su; Azaj Ahmad; Yao-Ching Hung; Wei-Chun Chang
Journal:  J Cell Mol Med       Date:  2020-06-16       Impact factor: 5.310

10.  Formation and characterization of lipid droplets of the bovine corpus luteum.

Authors:  Heather A Talbott; Michele R Plewes; Crystal Krause; Xiaoying Hou; Pan Zhang; William B Rizzo; Jennifer R Wood; Andrea S Cupp; John S Davis
Journal:  Sci Rep       Date:  2020-07-09       Impact factor: 4.996
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