Verônica Chomiski1, Alfredo Gragnani2, Jéssica Bonucci3, Silvana Aparecida Alves Correa4, Samuel Marcos Ribeiro de Noronha5, Lydia Masako Ferreira6. 1. Fellow MSc degree, Division of Plastic Surgery, Department of Surgery, Universidade Federal de São Paulo (UNIFESP0, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures, manuscript writing. 2. PhD, Associate Professor, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; manuscript writing; critical revision. 3. Fellow MSc degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures. 4. PhD, Postdoctoral degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures, acquisition of data, manuscript writing. 5. PhD, Postdoctoral degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, manuscript writing. 6. Head, Full Professor, Division of Plastic Surgery, UNIFESP, Researcher 1A-CNPq, Director Medicine III-CAPES, Sao Paulo-SP, Brazil. Intellectual and scientific content of the study.
Abstract
PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes. CONCLUSION: There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes. CONCLUSION: There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.