| Literature DB >> 27576906 |
Christina Gebler1, Tim Lohoff1, Maciej Paszkowski-Rogacz1, Jovan Mircetic1, Debojyoti Chakraborty1, Aylin Camgoz1, Martin V Hamann1, Mirko Theis1, Christian Thiede2,3, Frank Buchholz1,4,3,5.
Abstract
Although whole-genome sequencing has uncovered a large number of mutations that drive tumorigenesis, functional ratification for most mutations remains sparse. Here, we present an approach to test functional relevance of tumor mutations employing CRISPR/Cas9. Combining comprehensive sgRNA design and an efficient reporter assay to nominate efficient and selective sgRNAs, we establish a pipeline to dissect roles of cancer mutations with potential applicability to personalized medicine and future therapeutic use.Entities:
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Year: 2016 PMID： 27576906 PMCID： PMC6284257 DOI： 10.1093/jnci/djw183
Source DB: PubMed Journal: J Natl Cancer Inst ISSN： 0027-8874 Impact factor: 13.506